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PE/Cyanine7 anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK1dot5_PECy7_030206
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, ? PE/Cyanine7 isotype control (open histogram).
  • GK1dot5_PECy7_030206
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, ? PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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100421 25 µg 71€
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100422 100 µg 172€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  2. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  3. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  4. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  5. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  6. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  7. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  8. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  9. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  10. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  11. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  12. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  13. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  14. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  15. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  16. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  17. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  18. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  19. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  20. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  21. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  22. Diao L, et al. 2022. iScience. 25:105511. PubMed
  23. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  24. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  25. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  26. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  27. Kaufmann U et al. 2019. Cell metabolism. 29(5):1104-1118 . PubMed
  28. Matsuoka S, et al. 2019. Haematologica. 105:226. PubMed
  29. Rolla S, et al. 2010. J Immunol. 184:6124. PubMed
  30. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  31. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  32. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  33. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  34. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  35. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  36. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  37. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  38. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  39. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  40. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  41. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  42. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  43. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  44. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  45. An J, et al. 2022. iScience. 25:103570. PubMed
  46. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  47. Ohmichi Y, et al. 2011. J Exp Med . 208:1015. PubMed
  48. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  49. Cha SE, et al. 2021. Oncoimmunology. 10:1899469. PubMed
  50. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  51. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  52. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  53. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  54. Akhter N, et al. 2016. J Biol Chem. 291: 23672 - 23680. PubMed
  55. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  56. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  57. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  58. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  59. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  60. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  61. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  62. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  63. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  64. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  65. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  66. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  67. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  68. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  69. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  70. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  71. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  72. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  73. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  74. XS R, et al. 2015. Diabetes. 64 90. PubMed
  75. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  76. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  77. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  78. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  79. Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
  80. Busse DC, et al. 2020. J Virol. 94:00:00. PubMed
  81. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  82. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  83. Cui Z, et al. 2021. Cell Death Dis. 12:775. PubMed
  84. Kitajima Y, et al. 2022. Cells. 11:. PubMed
  85. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  86. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  87. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  88. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  89. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  90. Wang W, et al. 2021. STAR Protocols. 2(3):100756. PubMed
  91. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  92. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  93. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  94. Li C, et al. 2018. Cell. 174:285. PubMed
  95. Nakornpakdee Y, et al. 2018. Asian Pac J Allergy Immunol. 36:265. PubMed
  96. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  97. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  98. Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed
  99. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  100. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  101. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  102. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  103. Prüss H, et al. 2017. Nat Neurosci. 10.1038/nn.4643. PubMed
  104. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  105. Kelley WJ, et al. 2022. JCI Insight. :. PubMed
  106. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  107. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  108. Ambrosino E, et al. 2006. Cancer Res . 66:7734. PubMed
  109. Nagamata S, et al. 2019. Sci Rep. 9:3911. PubMed
  110. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  111. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  112. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  113. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  114. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  115. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  116. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  117. Kim YS, et al. 2018. Front Immunol. 9:1093. PubMed
  118. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  119. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  120. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  121. Simula L, et al. 2022. Mol Oncol. 16:188. PubMed
  122. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  123. Groves HT, et al. 2020. MBio. 11:e03236-19. PubMed
  124. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  125. Yu Z, et al. 2021. Biomed Res Int. 2021:9958745. PubMed
  126. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_312706 (BioLegend Cat. No. 100421)
AB_312707 (BioLegend Cat. No. 100422)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC,IHC-F,ICC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,ICC,SB
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD4

  • Biotin anti-mouse CD4

  • FITC anti-mouse CD4

  • PE anti-mouse CD4

  • PE/Cyanine5 anti-mouse CD4

  • Purified anti-mouse CD4

  • PE/Cyanine7 anti-mouse CD4

  • APC/Cyanine7 anti-mouse CD4

  • Alexa Fluor® 647 anti-mouse CD4

  • Alexa Fluor® 488 anti-mouse CD4

  • Pacific Blue™ anti-mouse CD4

  • Alexa Fluor® 700 anti-mouse CD4

  • PerCP anti-mouse CD4

  • PerCP/Cyanine5.5 anti-mouse CD4

  • Brilliant Violet 421™ anti-mouse CD4

  • Ultra-LEAF™ Purified anti-mouse CD4

  • Alexa Fluor® 594 anti-mouse CD4

  • Brilliant Violet 711™ anti-mouse CD4

  • Brilliant Violet 510™ anti-mouse CD4

  • Brilliant Violet 605™ anti-mouse CD4

  • Brilliant Violet 785™ anti-mouse CD4

  • PE/Dazzle™ 594 anti-mouse CD4

  • APC/Fire™ 750 anti-mouse CD4

  • GoInVivo™ Purified anti-mouse CD4

  • Brilliant Violet 750™ anti-mouse CD4

  • Brilliant Violet 650™ anti-mouse CD4

  • Spark Blue™ 550 anti-mouse CD4

  • Spark NIR™ 685 anti-mouse CD4

  • KIRAVIA Blue 520™ anti-mouse CD4

  • PE/Fire™ 640 anti-mouse CD4

  • APC/Fire™ 810 anti-mouse CD4

  • PE/Fire™ 700 anti-mouse CD4

  • Spark Violet™ 538 anti-mouse CD4

  • Spark YG™ 593 anti-mouse CD4

  • Spark Blue™ 574 anti-mouse CD4 Antibody

  • Spark UV™ 387 anti-mouse CD4
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