APC/Cyanine7 anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
8 reviews
Product Citations
publications
53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • 53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
100713 25 µg 68€
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100714 100 µg 156€
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions. The solution is free of unconjugated APC/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, and immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 μm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
Product Citations
  1. Chen J, et al. 2008. Blood. 111:5163. PubMed
  2. Shoji M, et al. 2012. PLoS One. 7:e30302. PubMed
  3. Kastenmüller K, et al. 2013. Infect Immun . 81:789. PubMed
  4. Spranger S, et al. 2013. Sci Transl Med. 26:200. PubMed
  5. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  6. Li J, et al. 2013. Arch Immunol Ther Exp (Warsz). 61:237. PubMed
  7. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  8. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  9. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  10. Gough D, et al. 2014. Blood. 124:2252. PubMed
  11. Singhal A, et al. 2014. Sci Transl Med. 6:263. PubMed
  12. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  13. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  14. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  15. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  16. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  17. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  18. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  19. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  20. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  21. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  22. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  23. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  24. Cha JH 2018. Molecular cell. 71:606. PubMed
  25. Bouziat R 2018. Cell host & microbe. 24:677. PubMed
  26. Snell LM 2018. Immunity. 49:678. PubMed
  27. Lian G 2018. eLife. 7: e36158. PubMed
  28. Turner JS, et al. 2018. Cell Rep. 25:1395. PubMed
  29. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  30. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  31. Herndler–Brandstetter D, et al. 2018. Immunity. 48:716. PubMed
  32. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  33. Miska J, et al. 2019. Cell Rep. 27:226. PubMed
  34. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  35. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  36. Das S, et al. 2019. Oncogene. 38:3316. PubMed
  37. Yang BH, et al. 2019. Cell Rep. 27:3629. PubMed
  38. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  39. Cianciaruso C, et al. 2019. Cell Rep. 27:3062. PubMed
Publication Library
RRID
AB_312752 (BioLegend Cat. No. 100713)
AB_312753 (BioLegend Cat. No. 100714)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/30/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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