FITC anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2
C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE
  • 17A2
    C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE
See FITC spectral data
Cat # Size Price Quantity Avail. Save
100203 50 µg 24€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100204 500 µg 96€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Lesmeister M, et al. 2005. Reprod Biol Endocrinol. 29:74. PubMed
  2. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  3. Wu X, et al. 2012. Biochem Pharmacol. 84:1164. PubMed
  4. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  5. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
  6. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  7. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
  8. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  9. White C, et al. 2014. J Immunol. 193:5933. PubMed
  10. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  11. Huang B, et al. 2015. PLoS One. 10: 0134715. PubMed
  12. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  13. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  14. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  15. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  16. Mingozzi F, et al. 2016. EMBO Mol Med. 8: 1039 - 1051. PubMed
  17. Chen Q, et al. 2016. Nat Commun. 7:13193. PubMed
  18. Akhter N, et al. 2016. J Biol Chem. 291: 23672 - 23680. PubMed
  19. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  20. Zan H, et al. 2017. Nat Commun. 8:14244. PubMed
  21. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  22. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  23. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  24. Wang X, et al. 2018. Mol Med Rep. 17:1660. PubMed
  25. Jia H, et al. 2018. Int J Oncol. 53:949. PubMed
  26. Solanki A, et al. 2018. Development. 145. PubMed
  27. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  28. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  29. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  30. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  31. Shields BD, et al. 2019. Cancer Res. 79:1113. PubMed
  32. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  33. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  34. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  35. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  36. Chen X, et al. 2019. Med Sci Monit. 25:5389. PubMed
  37. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  38. Li W, et al. 2019. Nat Commun. 10:3349. PubMed
  39. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  40. Lee JS et al. 2018. Cell. 174(6):1559-1570 . PubMed
  41. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  42. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  43. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  44. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  45. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  46. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  47. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  48. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
  49. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
  50. Xu X, et al. 2018. Cell. 175:1336. PubMed
  51. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  52. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  53. Roy S, et al. 2019. Front Immunol. 2.404166667. PubMed
  54. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  55. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  56. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  57. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  58. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  59. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  60. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  61. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  62. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  63. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  64. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  65. Pan X, et al. 2019. BMC Complement Altern Med. 19:163. PubMed
  66. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  67. Martínez-Vicente P, et al. 2019. PLoS Pathog. 15:e1007658. PubMed
  68. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  69. Lin W, et al. 2020. Theranostics. 10:4871. PubMed
  70. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
RRID
AB_312660 (BioLegend Cat. No. 100203)
AB_312661 (BioLegend Cat. No. 100204)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account