Brilliant Violet 750 anti-mouse CD3 Antibody anti-CD3

Pricing & Availability

Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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Product Citations: publications

17A2_BV750_CD3_Antibody_042618 — C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) Brilliant Violet 750™ (closed histogram). Open histogram represents unstained cells.

Compare all formats See Brilliant Violet 750™ spectral data

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100249

50 µg

238€

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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References (PubMed link indicates BioLegend citation)

Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Sanmarco LM, et al. 2021. Nature. 590:473. PubMed

RRID

AB_2734148 (BioLegend Cat. No. 100249)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

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Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD3	17A2	FC
PE anti-mouse CD3	17A2	FC
Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Alexa Fluor® 488 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3	17A2	FC
Alexa Fluor® 700 anti-mouse CD3	17A2	FC
PerCP/Cyanine5.5 anti-mouse CD3	17A2	FC
PE/Cyanine7 anti-mouse CD3	17A2	FC
APC/Cyanine7 anti-mouse CD3	17A2	FC
Brilliant Violet 421™ anti-mouse CD3	17A2	FC,ICC
Brilliant Violet 570™ anti-mouse CD3	17A2	FC
Brilliant Violet 650™ anti-mouse CD3	17A2	FC
Brilliant Violet 785™ anti-mouse CD3	17A2	FC
Brilliant Violet 510™ anti-mouse CD3	17A2	FC
APC anti-mouse CD3	17A2	FC
Ultra-LEAF™ Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3	17A2	FC
Alexa Fluor® 594 anti-mouse CD3	17A2	IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3	17A2	FC
Biotin anti-mouse CD3	17A2	FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3	17A2	FC
APC/Fire™ 750 anti-mouse CD3	17A2	FC
Brilliant Violet 750™ anti-mouse CD3	17A2	FC
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2	FC
Spark NIR™ 685 anti-mouse CD3	17A2	FC
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2	FC
PE/Fire™ 640 anti-mouse CD3	17A2	FC
Spark YG™ 570 anti-mouse CD3	17A2	IHC-F
PE/Fire™ 700 anti-mouse CD3	17A2	FC
PE/Cyanine5 anti-mouse CD3	17A2	FC
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2	FC
Spark Violet™ 423 anti-mouse CD3	17A2	FC
PE/Fire™ 810 anti-mouse CD3	17A2	FC
Spark Red™ 718 anti-mouse CD3	17A2	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/27/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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