PE anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PE_021606
C57BL/6 mouse splenocytes stained with 17A2 PE
  • 17A2_PE_021606
    C57BL/6 mouse splenocytes stained with 17A2 PE
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100205 50 µg 49€
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100206 200 µg 115€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  2. Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.071. PubMed
  3. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  4. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  5. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  6. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  7. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  8. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  9. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  10. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  11. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  12. Ushijima M, et al. 2018. Front Immunol. 9:243. PubMed
  13. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  14. Bedi B, et al. 2012. Proc Natl Acad Sci U S A. 109:275. PubMed
  15. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  16. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  17. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  18. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  19. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  20. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  21. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  22. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  23. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  24. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  25. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  26. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  27. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  28. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  29. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  30. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  31. Schmidt D, et al. 2016. PLoS One. 11: 0159059. PubMed
  32. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  33. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  34. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  35. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  36. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  37. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  38. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  39. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  40. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  41. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  42. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  43. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  44. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  45. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  46. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  47. Goldman E, et al. 2021. Cells. 10:. PubMed
  48. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  49. Wu J, et al. 2020. Immunity. 53:115. PubMed
  50. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  51. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  52. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  53. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  54. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  55. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  56. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  57. Hu J, et al. 2022. J Neuroinflammation. 19:71. PubMed
  58. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  59. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  60. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  61. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  62. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  63. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  64. Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed
  65. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  66. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  67. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  68. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  69. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  70. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  71. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  72. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  73. Pedraza-Zamora C, et al. 2017. Parasite Immunol. 10.1111/pim.12420. PubMed
  74. Huang Z, et al. 2021. Nat Commun. 12:145. PubMed
  75. Cho K, et al. 2021. iScience. 24:103117. PubMed
  76. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  77. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  78. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  79. Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed
  80. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  81. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  82. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  83. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  84. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  85. Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed
  86. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  87. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  88. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  89. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  90. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  91. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  92. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  93. Zhang T, et al. 2020. Cell. 180(6):1115-1129. PubMed
  94. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  95. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  96. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  97. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  98. Yamamura K, et al. 2017. Nat Commun. 8:13946. PubMed
  99. Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed
  100. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  101. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  102. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  103. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  104. Chen H, et al. 2022. Front Pharmacol. 13:827520. PubMed
  105. Schneider A, et al. 2014. J Leukoc Biol. 96:857. PubMed
  106. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  107. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  108. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  109. Zhou T, et al. 2021. Bioact Mater. 0.734027778. PubMed
  110. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  111. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  112. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  113. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  114. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  115. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  116. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  117. Fathman J, et al. 2010. Proc Natl Acad Sci U S A. 107:3663. PubMed
  118. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  119. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  120. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  121. Gawish R, et al. 2022. Elife. 11:. PubMed
  122. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  123. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  124. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  125. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  126. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  127. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
  128. Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
  129. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  130. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  131. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
RRID
AB_312662 (BioLegend Cat. No. 100205)
AB_312663 (BioLegend Cat. No. 100206)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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