- 17A2 (See other available formats)
- Other Names
- T cell antigen receptor complex, T3
- Rat IgG2b, κ
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- Product Citations
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CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.Product Details
- Antibody Type
- Host Species
- γδTCR-positive T-T hybridoma D1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-F - Quality tested
FC, 3-D IHC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.
(PubMed link indicates BioLegend citation)
- Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
- Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
- Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
- Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
- Zan H, et al. 2005. EMBO J. 24:3757.
- Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
- Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
- Konnecke I, et al. 2014. Bone. PubMed
- Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
- Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
- Product Citations
AB_2563427 (BioLegend Cat. No. 100240)
- Ig superfamily, CD3/TCR, 20 kD
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Antigen recognition, TCR signal transduction, T cell activation
- Peptide antigen/MHC-complex
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12502 View all products for this Gene ID
- View information about CD3 on UniProt.org
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