Alexa Fluor® 700 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_Alx700_020608
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
  • 30-F11_Alx700_020608
    C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
103127 25 µg 68€
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103128 100 µg 156€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  2. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
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  5. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  6. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  7. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  8. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
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  10. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  11. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  12. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  13. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  14. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  15. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  16. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  17. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  18. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  19. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  20. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  21. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  22. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  23. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  24. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  25. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  26. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  27. Acker KP, et al. 2019. iScience. 19:281. PubMed
  28. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  29. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  30. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  31. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  32. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  33. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  34. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  35. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  36. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  37. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
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  39. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
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  41. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  42. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  43. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
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  46. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  47. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  48. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  49. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  50. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  51. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  52. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  53. Li J, et al. 2018. Immunity. 49:178. PubMed
  54. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  55. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  56. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  57. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  58. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  59. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  60. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  61. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  62. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  63. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  64. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  65. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  66. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
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  69. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
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  73. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
RRID
AB_493714 (BioLegend Cat. No. 103127)
AB_493715 (BioLegend Cat. No. 103128)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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