Brilliant Violet 421™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_BV421_051911
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 421™ (filled histogram) or rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
  • 30-F11_BV421_051911
    C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 421™ (filled histogram) or rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
103133 125 µL 132€
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103134 50 µg 188€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Crncec I, et al. 2018. Mol Oncol. 12:514. PubMed
  2. Kramann R, et al. 2018. JCI Insight. 3. PubMed
  3. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  4. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  5. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  6. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  7. Durré T, et al. 2018. Nat Commun. 9:5178. PubMed
  8. Li Q, et al. 2019. Front Cell Neurosci. 12:520. PubMed
  9. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  10. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  11. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
  12. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  13. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  14. Shmeleva EV, et al. 2019. Front Immunol. 10:1780. PubMed
  15. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  16. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  17. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  18. Ulland TK et al. 2017. Cell. 170(4):649-663 . PubMed
  19. Mao W, et al. 2019. J Immunother Cancer. 0.484027778. PubMed
  20. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  21. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  22. Cullis J, et al. 2017. Cancer Immunol Res. 5:182. PubMed
  23. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  24. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  25. Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed
  26. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  27. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  28. Song WM, et al. 2018. J Exp Med. 215:745. PubMed
  29. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  30. Zahr A, et al. 2016. Nat Commun. 7:10363. PubMed
  31. Chu C, et al. 2016. Dis Model Mech. 9: 473-481. PubMed
  32. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  33. Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed
  34. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
RRID
AB_10899570 (BioLegend Cat. No. 103133)
AB_2562559 (BioLegend Cat. No. 103134)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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