FITC anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-f11
C57BL/6 splenocytes stained with 30-F11 FITC
  • 30-f11
    C57BL/6 splenocytes stained with 30-F11 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
103107 50 µg 24€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103108 500 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed
  2. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
  3. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  4. Saunders L, et al. 2015. Sci Transl Med. 7: 302ra136. PubMed
  5. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  6. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  7. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  8. J Gautam, X Zhang, Y Yao 2016. Sci Rep. 6:36450. PubMed
  9. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  10. Lim D, et al. 2018. Front Physiol. 9:47. PubMed
  11. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  12. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  13. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  14. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  15. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  16. Zhu H, et al. 2018. Elife. 7:. PubMed
  17. Xiao X, et al. 2019. Cell Discov. 5:2. PubMed
  18. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  19. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
  20. Padhye A, et al. 2019. Sci Rep. 9:4819. PubMed
  21. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  22. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  23. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  24. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  25. Poillet–Perez L, et al. 2018. Nature. 563:569. PubMed
  26. Plein A, et al. 2018. Nature. 562:223. PubMed
  27. Kobayashi S, et al. 2019. J Immunol. 203:1447. PubMed
  28. Hao T, et al. 2019. Nat Commun. 10:3665. PubMed
  29. Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed
  30. McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed
  31. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  32. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  33. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  34. Sarvi S et al. 2018. Cell chemical biology. 25(12):1456-1469 . PubMed
  35. Liu L et al. 2018. Cell stem cell. 23(4):544-556 . PubMed
  36. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  37. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  38. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  39. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  40. Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed
  41. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  42. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  43. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  44. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  45. Gastelum‐Aviña P et al. 2016. Journal of immunological methods. 438:42-50 . PubMed
  46. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  47. Maetzig T, et al. 2017. Mol Ther. 25:606. PubMed
  48. Al–Zaher AA, et al. 2018. Mol Ther Oncolytics. 0.376388889. PubMed
  49. Wan X, et al. 2018. Nature. 560:107. PubMed
  50. Shao Y, et al. 2019. Stem Cells. 37:1331. PubMed
  51. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  52. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  53. Yu JM, et al. 2019. Nat Commun. 4.388888889. PubMed
  54. Wang F, et al. 2019. MAbs. 12:1685350. PubMed
  55. Eckert EC, et al. 2020. Mol Ther Oncolytics. 0.710416667. PubMed
  56. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  57. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  58. Green DP, et al. 2019. Neuron. 101:412. PubMed
  59. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  60. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  61. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  62. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  63. Davidson S, et al. 2020. Cell Rep. 31:107628. PubMed
  64. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  65. Tsuchiya N, et al. 2019. Cell Rep. 29:162. PubMed
  66. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  67. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  68. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  69. Guarnerio J, et al. 2018. Nat Commun. 9:66. PubMed
  70. Xu X, et al. 2020. Chin Med. 15:33. PubMed
RRID
AB_312972 (BioLegend Cat. No. 103107)
AB_312973 (BioLegend Cat. No. 103108)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account