Multiomics and TotalSeq™ Reagents

Multiomics has transformed traditional sequencing experiments by providing unparalleled protein and genetic analysis on a single-cell or bulk sequencing basis. We offer comprehensive and unique products, including antibodies, panels, and hashtag reagents to enable protein detection by sequencing. Explore the capabilities of TotalSeq™ reagents and see how they seamlessly integrate into existing workflows to uncover what makes each cell unique.

TotalSeq™ format antibodies are patent pending proprietary technologies of BioLegend. Contact us if you are interested in partnering with us around this technology.

Comprehensive Solutions for Single-Cell and Bulk Multiomics

TotalSeq™ oligo-conjugated reagents enable protein detection by sequencing and integrate seamlessly into existing single-cell transcriptomics, bulk sequencing, or single-cell genomics workflows. Choose your experimental goal below and discover our solutions.

If you can’t find the reagents you’re looking for, contact us to discuss your project. We offer custom oligo-conjugated reagents including individual antibodies and cocktails. Let us know how we can support your multiomics work.

Single-Cell RNA and Protein Detection

Compatible with TotalSeq™-A, B, and C reagents.

Technologies like CITE-seq and REAP-seq utilize oligonucleotide-conjugated antibodies to simultaneously detect surface proteins and RNA. For this, we offer multiple formats of our TotalSeq™ antibodies, panels, and hashtags to incorporate into single-cell transcriptomics workflows, including Drop-Seq and those offered by 10x Genomics.

Explore Applications

Multiomics Analysis Software

For single-cell RNA and protein data analysis.

Processing and analysis of single-cell data can be complicated and require advanced tools. To simplify the process, BioLegend offers Multiomics Analysis Software (MAS), a free cloud-based program for the analysis of multiomics data. Featuring an intuitive user interface with flow cytometry-like gating, the software is accessible to all users irrespective of their bioinformatics background.

Analyze Data

Bulk RNA Sequencing

Compatible with TotalSeq™-A reagents.

Bulk RNA-sequencing is commonly used to analyze RNA expression from a population of cells in suspension. Our BEN-seq workflow, developed in collaboration with Illumina, stains cells in suspension with TotalSeq™ oligo-conjugated antibodies prior to bulk RNA sequencing, enabling the simultaneous profiling of cell surface proteins and RNA expression.

Expand Bulk Sequencing

Single-Cell DNA and Protein Detection

Compatible with TotalSeq™-D reagents.

Resolve complex genetic questions from genotype to phenotype by combining single-cell DNA analysis with protein detection using TotalSeq™-D oligo-conjugated reagents. Our TotalSeq™-D antibody cocktails are constructed for compatibility with the Mission Bio Tapestri platform to enable the co-detection of SNVs/indels, CNVs, and proteins.

Learn More

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Videos and Seminars

We’ve selected key introductory videos and seminars to help you get started and learn more about proteogenomics. For the most up-to-date information about upcoming webinars, visit our events page or sign-up for email updates.

View all videos

eBook/Application Notes

Download our eBook: The Evolution and Future of Single-Cell Proteogenomics to learn more about applications using TotalSeq™ reagents and how they can contribute to discoveries in precision oncology.

Download our application note: Correlated Expression of Protein and RNA Using Bulk and Single-Cell Proteogenomics to learn more about the utility of BEN-seq and see how we used the technique to detect 280 proteins and measure the Th1 response on both unstimulated and stimulated cells.

Protocols and Technical Resources

View our proteogenomics protocols for a complete list of protocols outlining how to use our TotalSeq™ reagents with sequencing workflows.

Need help deciding which TotalSeq™ reagents are right for you? Visit our frequently asked questions or contact our technical services team.

References

The list below contains selected publications referencing TotalSeq™ products, as well as the original CITE-seq (Stoeckius et al.) and REAP-seq (Peterson et al.) papers. Please note, manuscripts appearing in bioRxiv and medRxiv have not undergone a peer-review process.

Su Y, et al. Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19. Cell. 2020; October. DOI: 10.1016/j.cell.2020.10.037

Unterman A, et al. Single-Cell Omics Reveals Dyssynchrony of the Innate and Adapative Immune System in Progressive COVID-19. medRxiv. 2020; doi: 10.1101/2020.07.16.20153437

Muench DE, et al. Mouse models of neutropenia reveal progenitor stage-specific defects. Nature. 2020; Jun; 582 (7810): 109-114.

Arunachalam P, et al. T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers. Nat Med. 2020; doi: 10.1038/s41591-020-0858-8

Maier B, et al. A conserved dendritic-cell regulatory program limits antitumour immunity. Nature. 2020; doi: 10.1038/s41586-020-2134-y.

Kotliarov et al. Broad immune activation underlies shared set point signatures for vaccine responsiveness in healthy individuals and disease activity in patients with lupus. Nat Med. 2020; doi: 10.1038/s41591-020-0769-8.

Mulè M, et al. Normalizing and denoising protein expression data from droplet-based single cell profiling. bioRxiv. doi: 10.1101/2020.02.24.963603

Valenzi E, M, Tabib T, et al. Single-cell analysis reveals fibroblast heterogeneity and myofibroblasts in systemic sclerosis-associated interstitial lung disease. Annals of the Rheumatic Diseases 2019;78:1379-1387.

Granja et al. Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia. Nat Biotechnol. 2019 Dec; 37: 1458-1465.

Kendal et al. Identification of human tendon cell populations in healthy and diseased tissue using combined single cell transcriptiomics and proteomics. bioRxiv doi: 10.1101/2019.12.09/86993

Karagiannis et al. Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program. bioRxiv doi: 10.1101/630574

Gaublomme et al. Nuclei multiplexing with barcoded antibodies for single-nucleus genomics. Nat Communications. 2019 July; 10: 2907.

Stoeckius et al. Simultaneous epitope and transcriptome measurement in single cells. Nat Methods. 2017 Sep; 14(9): 865-868.

Peterson et al. Multiplexed quantification of proteins and transcripts in single cells. Nat Biotechnol. 2017 Oct; 35(10): 936-939.

Download and view antibody barcode information for all of our TotalSeq™ reagents. Check the boxes next to each antibody in your panel to export barcode information for only your antibodies of interest. Or, select download as excel file to download information for all of our products.