Multiomics and TotalSeq™ Reagents

Multiomics has transformed traditional sequencing experiments by providing unparalleled protein and genetic analysis on a single-cell or bulk sequencing basis. We offer comprehensive and unique products, including antibodies, panels, and hashtag reagents to enable protein detection by sequencing. Explore the capabilities of TotalSeq™ reagents and see how they seamlessly integrate into existing workflows to uncover what makes each cell unique.

TotalSeq™ format antibodies are patent pending proprietary technologies of BioLegend. Contact us if you are interested in partnering with us around this technology.

Comprehensive Solutions for Single-Cell and Bulk Multiomics

TotalSeq™ oligo-conjugated reagents enable protein detection by sequencing and integrate seamlessly into existing single-cell transcriptomics, bulk sequencing, or single-cell genomics workflows. Choose your experimental goal below and discover our solutions.

If you can’t find the reagents you’re looking for, contact us to discuss your project. We offer custom oligo-conjugated reagents including individual antibodies and cocktails. Let us know how we can support your multiomics work.

Single-Cell RNA and Protein Detection

Compatible with TotalSeq™-A, B, and C reagents.

Technologies like CITE-seq and REAP-seq utilize oligonucleotide-conjugated antibodies to simultaneously detect surface proteins and RNA. For this, we offer multiple formats of our TotalSeq™ antibodies, panels, and hashtags to incorporate into single-cell transcriptomics workflows, including Drop-Seq and those offered by 10x Genomics.

Explore Applications

Multiomics Analysis Software

For single-cell RNA and protein data analysis.

Processing and analysis of single-cell data can be complicated and require advanced tools. To simplify the process, BioLegend offers Multiomics Analysis Software (MAS), a free cloud-based program for the analysis of multiomics data. Featuring an intuitive user interface with flow cytometry-like gating, the software is accessible to all users irrespective of their bioinformatics background.

Analyze Data

Bulk RNA Sequencing

Compatible with TotalSeq™-A reagents.

Bulk RNA-sequencing is commonly used to analyze RNA expression from a population of cells in suspension. Our BEN-seq workflow, developed in collaboration with Illumina, stains cells in suspension with TotalSeq™ oligo-conjugated antibodies prior to bulk RNA sequencing, enabling the simultaneous profiling of cell surface proteins and RNA expression.

Expand Bulk Sequencing

Single-Cell DNA and Protein Detection

Compatible with TotalSeq™-D reagents.

Resolve complex genetic questions from genotype to phenotype by combining single-cell DNA analysis with protein detection using TotalSeq™-D oligo-conjugated reagents. Our TotalSeq™-D antibodies and cocktails are constructed for compatibility with the Mission Bio Tapestri platform to enable the co-detection of SNVs/indels, CNVs, and proteins.

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Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

New Research using TotalSeq™ Products

The list below contains selected publications referencing TotalSeq™ products, as well as the original CITE-seq (Stoeckius et al.) and REAP-seq (Peterson et al.) papers. Please note, manuscripts appearing in bioRxiv and medRxiv have not undergone a peer-review process.

Featured Publications

Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization.

Pombo Antunes AR, et al. Nat Neurosci. March 2021.

The authors used CITE-seq to map the glioblastoma myeloid cell landscape in mouse and human tumors. Their work revealed conserved dendritic cell and macrophage populations that were dynamic across disease stages.

Read paper >

Single-cell multi-omics analysis of the immune response in COVID-19.

Stephenson E, et al. Nat Medicine. April 2021.

Using a TotalSeq™-C antibody panel, the authors analyzed the surface proteome and T and B lymphocyte antigen receptors of patients with various severities of COVID-19. Their study describes a coordinated immune response and provides new insights for therapy.

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A conserved dendritic-cell regulatory program limits antitumour immunity.

Maier B, et al. Nature. 2020.

This study aimed to understand antitumor T cell immunity following PD-L1 blockade. Using single-cell multiomics, the authors identified a dendritic cell regulatory program that is induced by expression of IL-4 and limits antitumor activity.

Read paper >

All Publications

Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells.
Mimitou EP, et al. Nat Biotech. June 2021.
Integrated analysis of multimodal single-cell data.
Hao Y, et al. Cell. May 2021.
Single-cell multi-omics analysis of the immune response in COVID-19.
Stephenson E, et al. Nat Medicine. April 2021.

Characterizing the molecular regulation of inhibitory immune checkpoints with multimodal single-cell screens.
Papalexi E, et al. Nat Genetics. March 2021.
Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization.
Pombo Antunes AR, et al. Nat Neurosci. March 2021.
Maturation and persistence of the anti-SARS-CoV-2 memory B cell response.
Sokal A, et al. Cell. March 2021.

Simultaneous single cell measurements of intranuclear proteins and gene expression.
Chung H, et al. bioRxiv. Jan 2021.
Multi-modal Single-Cell Analysis Reveals Brain Immune Landscape Plasticity during Aging and Gut Microbiota Dysbiosis.
Golomb S, et al. Cell. Dec 2020.
TEA-seq: a trimodal assay for integrated single cell measurement of transcription, epitopes, and chromatin accessibility.
Swanson E, et al. bioRxiv. Nov 2020.
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.
Su Y, et al. Cell. Oct 2020
Single-Cell Omics Reveals Dyssynchrony of the Innate and Adapative Immune System in Progressive COVID-19.
Unterman A, et al. medRxiv. July 2020
Mouse models of neutropenia reveal progenitor stage-specific defects.
Muench DE, et al. Nature. June 2020.
T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers.
Arunachalam P, et al. Nat Med. 2020.
A conserved dendritic-cell regulatory program limits antitumour immunity.
Maier B, et al. Nature. 2020.
Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon.
Kendal et al. Scientific Reports. Sept 2020.

Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program.
Karagiannis et al. Nat Comm. May 2020.
Broad immune activation underlies shared set point signatures for vaccine responsiveness in healthy individuals and disease activity in patients with lupus.
Kotliarov et al. Nat Med. Feb 2020.
Normalizing and denoising protein expression data from droplet-based single cell profiling.
Mulè M, et al. bioRxiv. 2020.
Single-cell analysis reveals fibroblast heterogeneity and myofibroblasts in systemic sclerosis-associated interstitial lung disease.
Valenzi EM, et al. Annals of the Rheumatic Diseases 2019.
Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia.
Granja et al. Nat Biotechnol. Dec 2019.
Nuclei multiplexing with barcoded antibodies for single-nucleus genomics.
Gaublomme et al. Nat Communications. July 2019.
Simultaneous epitope and transcriptome measurement in single cells.
Stoeckius et al. Nat Methods. Sept 2017.
Multiplexed quantification of proteins and transcripts in single cells.
Peterson et al. Nat Biotechnol. Oct 2017.

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TotalSeq™ in Action: Video Protocols and Seminars

Using TotalSeq™ Antibodies for CITE-Seq

Walk through the CITE-seq workflow using TotalSeq™ antibodies, including cell surface labeling, GEM generation and barcoding, post-GEM cleanup, library amplification, and gene expression and cell surface protein library construction. See how these oligo-barcoded antibodies integrate seamlessly into single-cell RNA sequencing workflows available from 10x Genomics to uncover unique phenotypes and understand protein expression of 100+ targets on a single-cell level.

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Overview of the TotalSeq™ Workflow

TotalSeq™ reagents are designed to seamlessly integrate into the CITE-seq workflow and are compatible with most droplet-based single cell sequencing methods such as the 10x Genomics platform or Drop-seq. This video is a brief walk-through of the workflow to generate single cell proteomics and transcriptomics data.

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Introduction to Single Cell Proteogenomics Applications using TotalSeq™ Antibodies

BioLegend’s TotalSeq™ reagents enable simultaneous proteomics and transcriptomics analysis on the same cell using the CITE-seq workflow. Compatible with droplet-based single cell sequencing methods, TotalSeq™ uses antibody-oligo conjugates to measure specific cell surface proteins. This video provides an overview of the utilities of proteogenomics applications.

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Technical Resources

View our proteogenomics protocols for a complete list of protocols outlining how to use our TotalSeq™ reagents with sequencing workflows.

Need help deciding which TotalSeq™ reagents are right for you? Visit our frequently asked questions or contact our technical services team.

View all protocols >

Application Notes and eBooks

Application Note: Correlated Expression of Protein and RNA Using Bulk and Single-Cell Proteogenomics

Learn more about the utility of BEN-seq and see how we used the technique to detect 280 proteins and measure the Th1 response on both unstimulated and stimulated cells.

Download Application Note >

Ebook: The Evolution and Future of Single-Cell Proteogenomics

Learn more about applications using TotalSeq™ reagents and how they can contribute to discoveries in precision oncology.

Download Ebook >

Blogs

Tips and Tricks for Titrating TotalSeq™ Antibodies

Titrating TotalSeq™ antibodies for sequencing applications like CITE-seq is critical for optimal results. Read this blog post to understand the importance of titration, alternative titration methods, and the selection of antibody concentrations.

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Single-Cell Multiomics: A Beginner’s Guide to Understanding CITE-Seq and TotalSeq™ Reagents

Multiomics has transformed traditional sequencing experiments and can provide unparalleled protein and genetic analysis in a single experiment. Learn the basics behind techniques like CITE-seq and REAP-seq and discover how our TotalSeq™ reagents integrate into the workflow.

Read more >

Overview of Single-Cell Sequencing: Isolation Methods and Techniques

Discover how single-cell sequencing has propelled biology into a new era of discovery and learn about the methods that enable the isolation of single cells.

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Download and view antibody barcode information for all of our TotalSeq™ reagents. Check the boxes next to each antibody in your panel to export barcode information for only your antibodies of interest. Or, select download as excel file to download information for all of our products.