Features of MAS

Multiomics Analysis Software (MAS) is a free cloud-based program for exploring single-cell proteogenomics data generated using TotalSeq™ antibodies in conjunction with 10x Genomics' single-cell RNA workflows. Features of the software include:

• A simple user interface for exploring CITE-seq data.

• Accessible to all users irrespective of bioinformatics background.

• Accessible through your web browser, no server access required.

• Uses flow cytometry-like gating based on TotalSeq™ antibody staining.

• Creates UMAP, tSNE, or TriMAP dimensionality reduction plots.

• Easily share any workspace with collaborators or BioLegend Technical Services.

• Export project in *.h5ad format for use in other single-cell analysis programs.

To access MAS, request an account under the “Access the Software” tab. If you have an account, you can login or download the user guide.

What Can MAS Do?

Single-Cell Multiomics Workflow

Cells are stained with TotalSeq™ antibodies and processed through 10x Genomics' Feature Barcode workflow. Sequencing libraries are generated and sequenced on an Illumina platform.

Resulting sequencing data, either *.bcl or fastq files, are analyzed using Cell Ranger, which produces feature counts in the form of feature-barcode matrices. These matrices are uploaded into MAS for the exploration of single-cell proteogenomic data.

Getting Started with MAS

If you’d like to start exploring the software, but don’t have single-cell data to explore, download this dataset and scroll down to follow our video tutorial.

If you have a feature barcode matrix you’d like to explore, request an account under the "Access the Software" tab. Make sure the Features.tsv.gz file adheres to the following guidelines:

• Rows containing cell hashing antibodies, need to have “HTO_###” in column A and “Cell Hashing” listed in column C
• Rows containing non-cell hashing antibodies, need to have “ADT_###” in column A and “Antibody Capture” listed in column C

Recommended MAS Workflow