Single-Cell Proteogenomics to Analyze DNA and Protein
Antibody staining fits seamlessly into the existing Mission Bio workflow.
Stain Sample with TotalSeq™-D Antibodies

Target Amplification and Library Prep

Discover TotalSeq™-D Reagents

The TotalSeq™-D line of reagents is constructed for compatibility with the Mission Bio Tapestri platform. Each antibody is conjugated to an oligonucleotide that consists of a capture sequence, clone-specific barcode sequence, and a PCR handle. The PCR handle is used as a priming template for Mission Bio associated cell barcoding and primer oligonucleotides. TotalSeq™-D reagents are compatible with Illumina® sequencing platforms.
We offer both single TotalSeq-D™ antibody conjugates as well as our TotalSeq™-D Human Heme Oncology Cocktail v1.0. The Heme Oncology Cocktail contains 42 primary antibodies and 3 isotype control antibodies. Each antibody has been pre-titrated for optimal performance and the cocktail is provided in convenient, single-use tubes.
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Targets included in the panel:
CD1c
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CD10
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CD22
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CD45
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CD64
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CD138
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CD2
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CD11b
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CD25
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CD45RA
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CD69
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CD141
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CD3
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CD11c
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CD30
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CD45RO
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CD71
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CD163
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CD4
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CD13
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CD33
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CD49d
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CD83
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CD303
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CD5
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CD14
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CD34
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CD56
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CD90
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CD304
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CD7
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CD16
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CD38
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CD62L
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CD117
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FcεRIα
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CD8
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CD19
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CD44
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CD62P
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CD123
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HLA-DR
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Download the full list of antibody clones and barcodes.
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Representative Data
Read our application note to learn more about how BioLegend’s antibodies were used with the Mission Bio Tapestri platform to reveal novel correlations between genomic variants and protein expression in AML samples.

PBMCs from two donor samples were mixed together, stained with TotalSeq™️-D antibodies and run on Mission Bio's Tapestri platform. Heatmap visualization reveals the power to obtain genotype and phenotype from the same cells across thousands of cells.

Human PBMCs were stained with the TotalSeq™️-D Heme Oncology Cocktail v1.0 and processed using the Mission Bio Tapestri DNA and Protein workflow. Protein count data were transformed and visualized in a UMAP projection overlaid with protein. Clusters were identified based on protein expression only.
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