Resolve complex genetic questions from genotype to phenotype by combining single-cell genetic analysis with protein detection using TotalSeq™-D oligo-conjugated reagents. Single-cell sequencing overcomes many of the limitations presented by bulk-sequencing by increasing the sensitivity and enabling the resolution of different genotypes within a heterogenous sample, which is critical for understanding complex diseases like cancer. Resolution is increased by the addition of protein expression measurements and allows you to:

 

  • Perform simultaneous immunophenotyping to determine whether mutations are associated with specific cell types or states
  • Discover new targets
  • Link genotype to phenotype by co-detecting SNVs/indels, CNVs, and proteins simultaneously

 

Uncover genetic variations in heterogenous samples using Mission Bio’s Tapestri platform and add BioLegend’s TotalSeq™-D antibodies to correlate these mutations with protein expression.

Single-Cell Proteogenomics to Analyze DNA and Protein

 

Antibody staining fits seamlessly into the existing Mission Bio workflow.

 

Stain Sample with TotalSeq™-D Antibodies

Cell Encapsulation on
Mission Bio’s
Tapestri Platform

DNA and Protein Release

Cell Barcoding

Target Amplification and Library Prep

Sequence and Analysis

 

 

Discover TotalSeq™-D Reagents

 

The TotalSeq™-D line of reagents is constructed for compatibility with the Mission Bio Tapestri platform. Each antibody is conjugated to an oligonucleotide that consists of a capture sequence, clone-specific barcode sequence, and a PCR handle. The PCR handle is used as a priming template for Mission Bio associated cell barcoding and primer oligonucleotides. TotalSeq™-D reagents are compatible with Illumina® sequencing platforms.
 

We offer both single TotalSeq-D™ antibody conjugates as well as our TotalSeq™-D Human Heme Oncology Cocktail v1.0. The Heme Oncology Cocktail contains 42 primary antibodies and 3 isotype control antibodies. Each antibody has been pre-titrated for optimal performance and the cocktail is provided in convenient, single-use tubes.

 

Targets included in the panel:

 

CD1c

CD10

CD22

CD45

CD64

CD138

CD2

CD11b

CD25

CD45RA

CD69

CD141

CD3

CD11c

CD30

CD45RO

CD71

CD163

CD4

CD13

CD33

CD49d

CD83

CD303

CD5

CD14

CD34

CD56

CD90

CD304

CD7

CD16

CD38

CD62L

CD117

FcεRIα

CD8

CD19

CD44

CD62P

CD123

HLA-DR

Download the full list of antibody clones and barcodes.

 

Representative Data

 

Read our application note to learn more about how BioLegend’s antibodies were used with the Mission Bio Tapestri platform to reveal novel correlations between genomic variants and protein expression in AML samples.

 

 

 

 

TotalSeq™️-D antibodies and Mission Bio's Tapestri platform

PBMCs from two donor samples were mixed together, stained with TotalSeq™️-D antibodies and run on Mission Bio's Tapestri platform. Heatmap visualization reveals the power to obtain genotype and phenotype from the same cells across thousands of cells.

 

 

Human PBMCs were stained with the TotalSeq™️-D Heme Oncology Cocktail v1.0 and processed using the Mission Bio Tapestri DNA and Protein workflow. Protein count data were transformed and visualized in a UMAP projection overlaid with protein. Clusters were identified based on protein expression only.

 


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™️ products. For example, for any technology platform Buyer uses with TotalSeq™️, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™️ with that platform.

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