ELISA MAX™ Standard Set Mouse TNF-α

Pricing & Availability
Other Names
TNF-a ELISA Kit
Ave. Rating
19 reviews
Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Save
430901 5 plates 225 CHF
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. Kawamura K,et al.2007.Biol Reprod.76:611.PubMed
  3. Tsujikawa K,et al.2007.Proc Natl Acad Sci USA.104:16702.PubMed
  4. Desy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  5. Sing A, et al. 2011. Infect Immun. 79:4533. PubMed
  6. O'Meara TR, et al. 2013. MBio. 4:e00522. PubMed
  7. Pustelny C, et al. 2015. Infect Immun. 83:417. PubMed
  8. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  9. Maruhashi T, et al. 2015. J Immunol. 194:5681.
Product Citations
  1. Tsujikawa K, et al. 2007. Proc Natl Acad Sci U S A. 104:16702. PubMed
  2. Kawamura K, et al. 2007. Biol Reprod. 76:611. PubMed
  3. Désy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  4. Singh A, et al. 2011. Infect Immun. 79:4533. PubMed
  5. Barberi T, et al. 2012. PLoS One. 7:e29808. PubMed
  6. O'Meara T, et al. 2013. MBio. 4:e00522. PubMed
  7. Pustelny C, et al. 2015. Infect Immun . 83:417. PubMed
  8. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  9. Maruhashi T, et al. 2015. J Immunol. 194:5681. PubMed
  10. Vargas-Muñiz J, et al. 2015. Fungal Genet Biol. 81: 41-51. PubMed
  11. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  12. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  13. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  14. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  15. Renshaw H, et al. 2016. Infect Immun . 84: 1556 - 1564. PubMed
  16. Li X, et al. 2016. J Leukoc Biol. 100: 581 - 588. PubMed
  17. Morzelle M, et al. 2016. PLoS One. 11:e0166123. PubMed
  18. Swee L, et al. 2016. Open Bio. 6: 160293. PubMed
  19. Yang J, et al. 2017. Sci Rep. 7:42723. PubMed
  20. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  21. Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed
  22. Ho DH, et al. 2018. Exp Neurobiol. 27:171. PubMed
  23. Frost PS, et al. 2019. Cell Death Dis. 10:323. PubMed
  24. Rother M, et al. 2018. Cell Host Microbe. 23:661. PubMed
  25. Uchimura T, et al. 2018. Immunity. 49:1049. PubMed
  26. Zhao Y, et al. 2018. Mol Cell. 69:505. PubMed
  27. Zhou B, et al. 2019. MBio. 10. PubMed
  28. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
Publication Library
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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