APC/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_APCCyanine7_CD19_Antibody_031819_updated.png
C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • 6D5_APCCyanine7_CD19_Antibody_031819_updated.png
    C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
115529 25 µg 95,00 CHF
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115530 100 µg 195,00 CHF
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  2. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  3. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  4. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  5. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  6. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  7. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  8. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  9. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  10. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  11. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  12. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  13. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  14. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  15. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
  16. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  17. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  18. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  19. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  20. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  21. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  22. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  23. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  24. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  25. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  26. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  27. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  28. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  29. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  30. Engblom C, et al. 2017. Science. 358:6367. PubMed
  31. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  32. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  33. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  34. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  35. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
  36. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  37. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  38. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  39. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  40. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  41. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  42. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  43. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  44. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  45. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  46. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  47. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  48. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  49. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  50. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  51. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  52. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  53. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  54. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
RRID
AB_830706 (BioLegend Cat. No. 115529)
AB_830707 (BioLegend Cat. No. 115530)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
Pacific Blue™ anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC, IHC-F
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC, CyTOF®, IHC-F
APC/Fire™ 750 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark YG™ 581 anti-mouse CD19 6D5 FC
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC, CyTOF®
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F, FC
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC, CyTOF®, IHC-F
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Go To Top Version: 3    Revision Date: 03.18.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Pacific Blue™ anti-mouse CD19

  • PerCP anti-mouse CD19

  • PerCP/Cyanine5.5 anti-mouse CD19

  • Brilliant Violet 421™ anti-mouse CD19

  • Brilliant Violet 650™ anti-mouse CD19

  • APC anti-mouse CD19

  • Biotin anti-mouse CD19

  • FITC anti-mouse CD19

  • PE anti-mouse CD19

  • PE/Cyanine5 anti-mouse CD19

  • Purified anti-mouse CD19

  • APC/Fire™ 750 anti-mouse CD19

  • Alexa Fluor® 488 anti-mouse CD19

  • Alexa Fluor® 647 anti-mouse CD19

  • PE/Dazzle™ 594 anti-mouse CD19

  • TotalSeq™-A0093 anti-mouse CD19

  • TotalSeq™-B0093 anti-mouse CD19

  • Spark YG™ 581 anti-mouse CD19

  • Brilliant Violet 570™ anti-mouse CD19

  • Brilliant Violet 510™ anti-mouse CD19

  • Purified anti-mouse CD19 (Maxpar® Ready)

  • Brilliant Violet 711™ anti-mouse CD19

  • Spark NIR™ 685 anti-mouse CD19

  • APC/Fire™ 810 anti-mouse CD19

  • Brilliant Violet 750™ anti-mouse CD19

  • PE/Fire™ 640 anti-mouse CD19 Antibody

  • Spark Blue™ 574 anti-mouse CD19 Antibody

  • PE/Cyanine7 anti-mouse CD19

  • Alexa Fluor® 700 anti-mouse CD19

  • Brilliant Violet 605™ anti-mouse CD19

  • Brilliant Violet 785™ anti-mouse CD19

  • TotalSeq™-C0093 anti-mouse CD19

  • Spark YG™ 570 anti-mouse CD19

  • Alexa Fluor® 594 anti-mouse CD19

  • Ultra-LEAF™ Purified anti-mouse CD19

  • Spark Blue™ 550 anti-mouse CD19

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