APC/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_APCCyanine7_CD19_Antibody_031819_updated.png
C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • 6D5_APCCyanine7_CD19_Antibody_031819_updated.png
    C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
115529 25 µg 112 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115530 100 µg 229 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  5. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
  6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  8. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  9. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  10. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  11. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  12. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  13. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  14. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
  15. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  16. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  17. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  18. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  19. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  20. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  21. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  22. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  23. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  24. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  25. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  26. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  27. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  28. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  29. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  30. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  31. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  32. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  33. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  34. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  35. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  36. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  37. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  38. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  39. Wei SC, et al. 2020. Cancer Discov. . PubMed
  40. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  41. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  42. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  43. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  44. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  45. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  46. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  47. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  48. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  49. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  50. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  51. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  52. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  53. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  54. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  55. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  56. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  57. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  58. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  59. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  60. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  61. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  62. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  63. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
  64. Elkhatib SK, et al. 2021. Biol Psychiatry Glob Open Sci. 1:190. PubMed
  65. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  66. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  67. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  68. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  69. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  70. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  71. Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  72. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  73. Engblom C, et al. 2017. Science. 358:6367. PubMed
  74. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  75. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  76. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  77. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  78. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  79. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  80. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  81. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  82. Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed
  83. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  84. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  85. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  86. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  87. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  88. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  89. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  90. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  91. Chavez JS, et al. 2022. Cells. 11:. PubMed
  92. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  93. Wilson CS, et al. 2021. JCI Insight. 6:. PubMed
  94. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  95. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  96. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  97. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  98. Cheng Q, et al. 2020. Eur J Immunol. 50:284. PubMed
  99. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
RRID
AB_830706 (BioLegend Cat. No. 115529)
AB_830707 (BioLegend Cat. No. 115530)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 03.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account