Alexa Fluor® 488 anti-mouse CD357 (GITR) Antibody

Pricing & Availability
YGITR 765 (See other available formats)
Regulatory Status
Other Names
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
BALB/c mouse splenocytes stained with PE anti-muse CD25 (PC61) and Alexa Fluor® 488 rat IgG2b, k isotype control (bottom) or Alexa Fluor® 488 YGITR 765 (upper)
  • YGITR_765
    BALB/c mouse splenocytes stained with PE anti-muse CD25 (PC61) and Alexa Fluor® 488 rat IgG2b, k isotype control (bottom) or Alexa Fluor® 488 YGITR 765 (upper)
  • YGITR_765_neg
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
120211 100 µg $295.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote

GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Recombinant mouse GITR protein and Transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 120216).

Application References

(PubMed link indicates BioLegend citation)
  1. Cobbold SP, et al. 2004. J. Immunol. 172:6003.
  2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
Product Citations
  1. Iype T, et al. 2010. J Immunol. 185:6115. PubMed
AB_439727 (BioLegend Cat. No. 120211)

Antigen Details

TNF receptor superfamily member
Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.
Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
GITR ligand (TNFSF18)
Cell Type
T cells, Tregs
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613
3. Kanamaru F, et al. 2004. J. Immunol. 172:613
4. Shimizu J, et al. 2002. Nat. Immunol. 3:135
5. Stephens GL, et al. 2004. J. Immunol. 173:5008
6. McHugh RS, et al. 2002. Immunity 16:311

Gene ID
21936 View all products for this Gene ID
View information about CD357 on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Remember me
Forgot your password? Reset Password
Request an Account