Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2

Pricing & Availability

Clone: RA3-6B2 (See other available formats)
Regulatory Status: RUO
Other Names: B220
Isotype: Rat IgG2a, κ
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Product Citations: publications

RA3-6B2_LEAF_090507 — BALB/c mouse splenocytes stained with Ultra-LEAF™ purified RA3-6B2, followed by anti-rat IgG FITC

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Size

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103269

100 µg

$193

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103270

1 mg

$605

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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cat

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF® - Verified
IHC-F, IHC-P, IP, Activ - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.

Application References (PubMed link indicates BioLegend citation)

Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
George A, et al. 1994. J. Immunol. 152:1014. (Activ)
Asensi V, et al. 1989. Immunology 68:204. (Activ)
Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Chang C L-T, et al. 2007. J. Immunol. 178:6984.
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Lang GL, et al. 2008. Blood 111:2158. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Formaglio P, et al. 2021. Immunity. 54:2724. PubMed

RRID

AB_2876406 (BioLegend Cat. No. 103269)
AB_2832305 (BioLegend Cat. No. 103270)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt: View information about CD45R on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F, FC, 3D IHC
APC anti-mouse/human CD45R/B220	RA3-6B2	FC
Biotin anti-mouse/human CD45R/B220	RA3-6B2	FC, IHC-F
FITC anti-mouse/human CD45R/B220	RA3-6B2	FC
PE anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Cyanine5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Purified anti-mouse/human CD45R/B220	RA3-6B2	FC, CyTOF®, IHC-F, IHC-P, IP, Activ
PE/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220	RA3-6B2	FC, IHC-F, 3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220	RA3-6B2	FC, IHC-F, 3D IHC, SB
Pacific Blue™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220	RA3-6B2	FC, IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220	RA3-6B2	FC, IHC-F, SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)	RA3-6B2	FC, CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 750 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Spark Blue™ 550 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark NIR™ 685 anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220	RA3-6B2	FC, CyTOF®, IHC-F, IHC-P, IP, Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
PE/Fire™ 640 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Fire™ 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Violet™ 538 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 581 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 570 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Red™ 718 anti-mouse/human CD45R/B220	RA3-6B2	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/03/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.