Alexa Fluor® 647 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
1_RA3-6B2_AF647_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 647
  • 1_RA3-6B2_AF647_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 647
  • 2_RA3-6B2_AF647_B220_Antibody_IHC_031914
    C57BL/6 mouse frozen lymph node sections were fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 594 (red), 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 647 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3_Still-image_0316-5_20X-1_1
    Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD3 (clone 17A2) Alexa Fluor® 488 (red), CD21/35 (CR2/CR1)(clone 7E9) Alexa Fluor® 594 (green), and CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 215 μm imaging depth on a confocal microscope.
See Alexa Fluor® 647 spectral data See high resolution IHC data...
Cat # Size Price Quantity Avail. Save
103229 25 µg $85
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103226 100 µg $195
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC, 3-D IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5-5 µg/ml is suggested. For immunofluorescence microscopy, a concentration range of 1.25-10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103216).

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Brownlie R, et al. 2008. J Exp Med. 205:883. PubMed
  2. Gil-Cruz C, et al. 2012. Proc Natl Acad Sci U S A. 109:1233. PubMed
  3. Harumiya S, et al. 2013. Arch Biochem Biophys. 533:18. PubMed
  4. Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed
  5. Medgyesi D, et al. 2014. J Exp Med. 211:427. PubMed
  6. Neubert K, et al. 2014. J Immunol. 192:5830. PubMed
  7. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  8. Kang S, et al. 2016. J Immunol. 196: 196 - 206. PubMed
  9. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  10. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  11. Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed
  12. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  13. Lin JR 2018. eLife. 7: e31657. PubMed
  14. Beura LK 2018. Immunity. 48:327. PubMed
  15. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  16. Ramakrishnan SK, et al. 2019. Nat Commun. 10:660. PubMed
  17. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
Publication Library
RRID
AB_492875 (BioLegend Cat. No. 103229)
AB_389330 (BioLegend Cat. No. 103226)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 06/27/2014

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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