Pacific Blue™ anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RA3-6B2_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
  • RA3-6B2_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
103230 25 µg $94
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103227 100 µg $215
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  2. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  3. Schmid C, et al. 2015. J Exp Med. 212:775. PubMed
  4. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  5. Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed
  6. Makdasi E, et al. 2009. J Immunol. 182:6143. PubMed
  7. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  8. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  9. Grosjean F, et al. 2015. PLoS One. 10: 0133917. PubMed
  10. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  11. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  12. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  13. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  14. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  15. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  16. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  17. Kat I, et al. 2010. Int Immunol. 0.986805556. PubMed
  18. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  19. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  20. Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed
  21. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  22. Simerska P, et al. 2014. Vaccine. 32:4743. PubMed
  23. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  24. Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed
  25. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  26. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  27. Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed
  28. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
  29. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  30. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  31. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  32. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  33. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  34. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  35. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  36. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  37. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  38. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  39. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  40. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
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  42. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  43. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  44. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
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  46. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  47. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  48. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  49. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  50. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  51. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  52. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  53. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  54. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  55. Nishimori J, et al. 2012. Infect Immun . 80:4398. PubMed
  56. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  57. Ilsley M, et al. 2017. Nucleic Acids Research. 10.1093/nar/gkx441. PubMed
  58. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  59. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  60. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  61. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
  62. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  63. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  64. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  65. Smith LK, et al. 2021. Elife. 10:. PubMed
  66. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  67. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  68. Eilat E 2013. J Immunol. 190:1472. PubMed
  69. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  70. Fast EM, et al. 2021. Elife. 10:. PubMed
  71. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  72. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  73. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
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  76. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
RRID
AB_492877 (BioLegend Cat. No. 103230)
AB_492876 (BioLegend Cat. No. 103227)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F, FC, 3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC, IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC, CyTOF®, IHC-F, IHC-P, IP, Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC, IHC-F, 3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC, IHC-F, 3D IHC, SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC, IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC, IHC-F, SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC, CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC, CyTOF®, IHC-F, IHC-P, IP, Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 594 anti-mouse/human CD45R/B220

  • APC anti-mouse/human CD45R/B220

  • Biotin anti-mouse/human CD45R/B220

  • FITC anti-mouse/human CD45R/B220

  • PE anti-mouse/human CD45R/B220

  • PE/Cyanine5 anti-mouse/human CD45R/B220

  • Purified anti-mouse/human CD45R/B220

  • PE/Cyanine7 anti-mouse/human CD45R/B220

  • APC/Cyanine7 anti-mouse/human CD45R/B220

  • Alexa Fluor® 488 anti-mouse/human CD45R/B220

  • Alexa Fluor® 647 anti-mouse/human CD45R/B220

  • Pacific Blue™ anti-mouse/human CD45R/B220

  • Alexa Fluor® 700 anti-mouse/human CD45R/B220

  • PerCP anti-mouse/human CD45R/B220

  • PerCP/Cyanine5.5 anti-mouse/human CD45R/B220

  • Brilliant Violet 421™ anti-mouse/human CD45R/B220

  • Brilliant Violet 570™ anti-mouse/human CD45R/B220

  • Brilliant Violet 650™ anti-mouse/human CD45R/B220

  • Brilliant Violet 605™ anti-mouse/human CD45R/B220

  • Brilliant Violet 785™ anti-mouse/human CD45R/B220

  • Brilliant Violet 510™ anti-mouse/human CD45R/B220

  • Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)

  • Brilliant Violet 711™ anti-mouse/human CD45R/B220

  • PE/Dazzle™ 594 anti-mouse/human CD45R/B220

  • APC/Fire™ 750 anti-mouse/human CD45R/B220

  • Brilliant Violet 750™ anti-mouse/human CD45R/B220

  • TotalSeq™-A0103 anti-mouse/human CD45R/B220

  • Spark Blue™ 550 anti-mouse/human CD45R/B220

  • Spark NIR™ 685 anti-mouse/human CD45R/B220

  • TotalSeq™-B0103 anti-mouse/human CD45R/B220

  • Ultra-LEAF™ Purified anti-mouse/human CD45R/B220

  • TotalSeq™-C0103 anti-mouse/human CD45R/B220

  • PE/Fire™ 640 anti-mouse/human CD45R/B220

  • APC/Fire™ 810 anti-mouse/human CD45R/B220

  • PE/Fire™ 700 anti-mouse/human CD45R/B220

  • Spark Violet™ 538 anti-mouse/human CD45R/B220

  • Spark YG™ 581 anti-mouse/human CD45R/B220

  • Spark YG™ 570 anti-mouse/human CD45R/B220

  • PE/Fire™ 810 anti-mouse/human CD45R/B220

  • Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody

  • Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody

  • Spark Red™ 718 anti-mouse/human CD45R/B220

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