Purified anti-rat CD28 Antibody

Pricing & Availability
JJ319 (See other available formats)
Regulatory Status
Other Names
Tp44, T44
Mouse IgG1, κ
Ave. Rating
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Product Citations
LOU rat splenocytes stained with purified JJ319, followed by anti-mouse IgG FITC
  • JJ319_Pure_021308
    LOU rat splenocytes stained with purified JJ319, followed by anti-mouse IgG FITC
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200902 500 µg $237
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CD28 is a 44 kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, α/βTCR+ T cells, most γ/δTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation. 

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Rat CD28-transfected BALB/c mouse A2OJ cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The JJ319 antibody has been reported to augment in vitro T cell proliferation and enhance IL-2 production. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of proliferation responses1, and in vitro costimulation of IL-2 production in the presence of R73 antibody (anti-rat a/ß TCR, Cat. No. 201112)2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tacke M, et al. 1995. J. Immunol. 154:5121. (Costim)
  2. Tacke M, et al. 1997. Eur. J. Immunol. 27:239. (Costim)
  3. Du Y, et al. 2012. PLoS One. 7:e52911. PubMed.
Product Citations
  1. Du Y, et al. 2012. PLoS One. 7:e52911. PubMed
AB_313891 (BioLegend Cat. No. 200902)

Antigen Details

Ig superfamily, 44 kD

All α/βTCR+ T cells, most γ/δTCR+ T cells, NK cell subset

T cell costimulation
CD80 (B7-1) and CD86 (B7-2)
Cell Type
T cells, NK cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Tacke M, et al. 1995. J. Immunol. 154:5121.
2. Mitnancht R, et al. 1995. Eur. J. Immunol. 25:328.
3. Tacke M, et al. 1997. Eur. J. Immunol. 27:239.

Gene ID
25660 View all products for this Gene ID
View information about CD28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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