Purified anti-rat CD3 Antibody

Pricing & Availability
Clone
1F4 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Mouse IgM, κ
Ave. Rating
Submit a Review
Product Citations
publications
1F4_Pure_021009.jpg
LOU rat splenocytes stained with purified 1F4, followed by anti-mouse Ig FITC
  • 1F4_Pure_021009.jpg
    LOU rat splenocytes stained with purified 1F4, followed by anti-mouse Ig FITC
Cat # Size Price Quantity Avail. Save
201401 100 µg $85
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. 

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
  2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
  3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
  4. Baba T, et al. 2006. Blood 107:2004. (IHC)
  5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
  6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
RRID
AB_893302 (BioLegend Cat. No. 201401)

Antigen Details

Structure
Ig superfamily, approximately 20-25 kD
Distribution

Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC complex
Cell Type
NKT cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.

Gene ID
25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account