APC anti-rat CD3 Antibody

Pricing & Availability
1F4 (See other available formats)
Regulatory Status
Other Names
T cell antigen receptor complex, T3
Mouse IgM, κ
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Product Citations
Lewis rat splenocytes were stained with CD3 (clone 1F4) APC (filled histogram) or mouse IgM, κ APC isotype control (open histogram).
  • 1F4_APC_CD3_Antibody_FC_032514.jpg
    Lewis rat splenocytes were stained with CD3 (clone 1F4) APC (filled histogram) or mouse IgM, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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201413 25 µg $72
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201414 100 µg $193
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CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
F344 rat spleen cells stimulated with PMA and calcium ionophore
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
  2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
  3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
  4. Baba T, et al. 2006. Blood 107:2004. (IHC)
  5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
  6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
Product Citations
  1. Zimmerman KA, et al. 2019. J Am Soc Nephrol. 30:767. PubMed
AB_2563365 (BioLegend Cat. No. 201413)
AB_2563366 (BioLegend Cat. No. 201414)

Antigen Details

Ig superfamily, approximately 20-25 kD

Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells

Antigen recognition, TCR signal transduction, T cell activation
Peptide antigen/MHC complex
Cell Type
NKT cells, T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.

Gene ID
25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Ultra-LEAF™ Purified anti-rat CD3

  • PerCP/Cyanine5.5 anti-rat CD3


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