- Clone
- 1F4 (See other available formats)
- Other Names
- T cell antigen receptor complex, T3
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Lewis rat splenocytes were stained with CD3 (clone 1F4) PE (filled histogram) or mouse IgM, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 201411 | 25 µg | $75 | ||||
| 201412 | 100 µg | $135 | ||||
CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
Product DetailsProduct Details
- Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- F344 rat spleen cells stimulated with PMA and calcium ionophore
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.
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Application References
(PubMed link indicates BioLegend citation) -
- Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
- Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
- Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
- Baba T, et al. 2006. Blood 107:2004. (IHC)
- Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
- Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
- Product Citations
- RRID
-
AB_2563272 (BioLegend Cat. No. 201411)
AB_2563273 (BioLegend Cat. No. 201412)
Antigen Details
- Structure
- Ig superfamily, approximately 20-25 kD
- Distribution
-
Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC complex
- Cell Type
- NKT cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. - Gene ID
- 25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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