Purified anti-PSD95 Antibody (Previously Covance catalog# MMS-5183)

Pricing & Availability
Clone
K28/74 (See other available formats)
Other Names
Disks large homolog 4, discs large homolog 4, Tax interaction protein 15, synapse-associated protein 90, postsynaptic density protein 95, post-synaptic density protein 95
Previously
Covance Catalog# MMS-5183
Isotype
Mouse IgG1
Ave. Rating
2 reviews
Product Citations
publications
A_K28slash74_PURE_PSD95_WB_021218
Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_K28slash74_PURE_PSD95_WB_021218
    Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_K28slash74_PURE_PSD95_Mu_IHCP_021218
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_K28slash74_PURE_PSD95_Rat_IHCP_021218
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D_K28slash74_PURE_PSD95_Hu_IHCP_021218
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • E_K28slash74_PURE_PSD95_Mu_IHCP_1_070218
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • F_K28slash74_PURE_PSD95_Mu_IHCP_2_070218
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
810302 25 µl $70.00
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810301 100 µl $140.00
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Description

PSD95, also known as SAP-90, is a member of the MAGUK family, encoded by the the DLG4 gene.  At postsynaptic sites, it mediates the scaffolding of NMDA receptors, AMPA receptors, potassium channels, and other associated signaling protein clusters. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 5.0 µg/ml. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections (IHC-P).

RRID
AB_2734563 (BioLegend Cat. No. 810302)
AB_2564749 (BioLegend Cat. No. 810301)

Related FAQs

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Go To Top Version: 3    Revision Date: 02/12/2018

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