Purified anti-SNAP-25 Antibody

Pricing & Availability
Clone
SMI 81 (See other available formats)
Other Names
Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype
Mouse IgG1, κ
Ave. Rating
3 reviews
Product Citations
publications
SMI-81_Pure_SNAP25_Antibody_1_081718
IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_1_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_2_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_3_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_4_081718
    Western blot of purified anti-SNAP-25 antibody (clone SMI 81). Lane 1: Molecular weight marker; Lane 2: 15 µg of human brain lysate; Lane 3: 15 µg of mouse brain lysate; Lane 4: 15 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
836303 25 µg $95
Check Availability


Need larger quantities of this item?
Request Bulk Quote
836304 100 µg $235
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length, unaltered SNAP-25.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated
EM, FC, IF, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
  2. Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
  3. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
  4. Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
  5. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
  6. Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
  7. Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
  8. Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
Product Citations
  1. Hirano A, et al. 2011. J Comp Neurol. 519:972-988. PubMed
  2. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  3. Brinkmalm A, et al. 2014. Mol Neurodegener. 9:53. PubMed
  4. Gu Y, et al. 2016. Proc Natl Acad Sci U S A. 113: 922 - 931. PubMed
  5. Kabachinski G, et al. 2016. Mol Biol Cell. 27: 654 - 668. PubMed
  6. Kunii M, et al. 2016. J Cell Biol. 215: 121 - 138. PubMed
Publication Library
RRID
AB_2715864 (BioLegend Cat. No. 836303)
AB_2566521 (BioLegend Cat. No. 836304)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus, and cell membrane.

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/13/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account