Purified anti-Syntaxin Antibody (Previously Covance catalog# MMS-619P)

Pricing & Availability
Clone
SP8 (See other available formats)
Other Names
Syntaxin-1A, neuron-specific antigen HPC-1
Previously
Covance Catalog# MMS-619P
Isotype
Mouse IgG1
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Product Citations
publications
1_SP8_PURE_Syntaxin_Antibody_WB_051818
Western blot of purified anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of clone SP8 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_SP8_PURE_Syntaxin_Antibody_WB_051818
    Western blot of purified anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of clone SP8 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_SP8_PURE_Syntaxin_Antibody_IHC_1_MB_051818
    IHC staining of purified anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 3_SP8_PURE_Syntaxin_Antibody_IHC_2_RB_051818
    IHC staining of purified anti-Syntaxin Antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • SP8_Purified_Syntaxin_IHC_020615
    IHC staining of purified anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
827002 25 µl $95
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827001 100 µl $234
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Description

Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a crude synaptic preparation from a post-mortem human brain.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P) and Western Blotting (WB).

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1997. Neuroscience 78(1):99-110.
  2. Honer WG, et al. 1993. Brain Res. 609(1-2):9-20.
RRID
AB_2749914 (BioLegend Cat. No. 827002)
AB_2564907 (BioLegend Cat. No. 827001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6804 View all products for this Gene ID
UniProt
View information about Syntaxin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/02/2018

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