PE anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RMP1-30
Con A (3-day) activated C57BL/6 mouse splenocytes stained with RMP1-30 PE
  • RMP1-30
    Con A (3-day) activated C57BL/6 mouse splenocytes stained with RMP1-30 PE
Compare all formats See PE spectral data
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109103 50 µg $122
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109104 200 µg $353
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  3. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  4. Bally AP, et al. 2017. J Immunol. 198:205. PubMed
  5. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  6. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  7. Tata A, et al. 2021. Oncoimmunology. 10:1933808. PubMed
  8. Bally A, et al. 2015. J Immunol. 194:4545. PubMed
  9. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  10. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  11. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  12. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  13. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  14. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  15. Nicolay N, et al. 2016. Sci Rep. 6: 26645. PubMed
  16. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  17. An J, et al. 2022. iScience. 25:103570. PubMed
  18. Zeng W, et al. 2016. Sci Rep. 6:36560. PubMed
  19. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  20. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  21. Karnowski A, et al. 2012. J Exp Med. 209:2049. PubMed
  22. Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed
  23. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  24. Park S, et al. 2014. J Leukoc Biol. 95:621. PubMed
  25. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  26. Li H, et al. 2020. Nature. 584:274. PubMed
  27. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  28. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  29. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  30. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  31. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  32. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  33. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  34. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  35. Puleston D, et al. 2014. Elife. 3:3706. PubMed
  36. Park H, et al. 2012. Cell Immunol. 278:76. PubMed
RRID
AB_313420 (BioLegend Cat. No. 109103)
AB_313421 (BioLegend Cat. No. 109104)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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