GoInVivo™ Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-14 (See other available formats)
Other Names
Programmed Death-1, CD279, PD 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP1-14_GoInVivo_CD279_Antibody_051217
Anti-mouse PD-1 inhibits the binding of mouse PD-1 to PD-L1. Immobilized PD-L1-Fc chimeric protein was incubated with PD-1 in the presence of anti-mouse PD-1 (clone RMP1-14, purple squares) or isotype control (clone RTK2758, purple circles). Bound PD-1 was detected with an HRP conjugate system. Clone RMP1-14 inhibits PD-L1/PD-1 interaction in a dose dependent manner.
  • RMP1-14_GoInVivo_CD279_Antibody_051217
    Anti-mouse PD-1 inhibits the binding of mouse PD-1 to PD-L1. Immobilized PD-L1-Fc chimeric protein was incubated with PD-1 in the presence of anti-mouse PD-1 (clone RMP1-14, purple squares) or isotype control (clone RTK2758, purple circles). Bound PD-1 was detected with an HRP conjugate system. Clone RMP1-14 inhibits PD-L1/PD-1 interaction in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
114114 5 mg $700
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114115 25 mg $2,150
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114116 50 mg $3,250
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114111 100 mg $4,850
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114112 500 mg $16,750
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114113 1 g $26,000
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.01 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography. The antibody is bottled at the concentration indicated on the vial, typically between 1 mg/ml and 3 mg/ml.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, WB, FA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
  2. Personal Communication (WB)
  3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
  4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
  5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
  7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
RRID
AB_2566278 (BioLegend Cat. No. 114114)
AB_2566279 (BioLegend Cat. No. 114115)
AB_2566280 (BioLegend Cat. No. 114116)
AB_2566089 (BioLegend Cat. No. 114111)
AB_2566090 (BioLegend Cat. No. 114112)
AB_2566091 (BioLegend Cat. No. 114113)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
Thymocytes, T cells, B cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Cancer Biomarkers, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11/30/2016

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