KIRAVIA Blue 520™ anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RMP130_KB520_CD279_Antibody_062422
Con A (three-days) activated C57BL/6 splenocytes were stained with anti-mouse CD3 APC/Cyanine7 and anti-mouse CD279 (PD-1) (clone RMP1-30) KIRAVIA Blue 520™ (left) or rat IgG2b, κ KIRAVIA Blue 520™ isotype control (right).
  • RMP130_KB520_CD279_Antibody_062422
    Con A (three-days) activated C57BL/6 splenocytes were stained with anti-mouse CD3 APC/Cyanine7 and anti-mouse CD279 (PD-1) (clone RMP1-30) KIRAVIA Blue 520™ (left) or rat IgG2b, κ KIRAVIA Blue 520™ isotype control (right).
Compare all formats See KIRAVIA Blue 520™ spectral data
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109129 25 µg $121
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109130 100 µg $380
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.


KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser
Blue Laser (488 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
RRID
AB_2924449 (BioLegend Cat. No. 109129)
AB_2924449 (BioLegend Cat. No. 109130)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse CD279 (PD-1)

  • Purified anti-mouse CD279 (PD-1)

  • Biotin anti-mouse CD279 (PD-1)

  • LEAF™ Purified anti-mouse CD279 (PD-1)

  • PE/Cyanine7 anti-mouse CD279 (PD-1)

  • APC anti-mouse CD279 (PD-1)

  • Purified anti-mouse CD279 (PD-1) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD279 (PD-1)

  • Alexa Fluor® 647 anti-mouse CD279 (PD-1)

  • PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)

  • Brilliant Violet 421™ anti-mouse CD279 (PD-1)

  • TotalSeq™-A0004 anti-mouse CD279 (PD-1)

  • TotalSeq™-B0004 anti-mouse CD279 (PD-1)

  • TotalSeq™-C0004 anti-mouse CD279 (PD-1)

  • KIRAVIA Blue 520™ anti-mouse CD279 (PD-1)

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