PE anti-human CD253 (TRAIL) Antibody

Pricing & Availability
RIK-2 (See other available formats)
Regulatory Status
Other Names
Apo-2 ligand, Apo-2L, TNFSF10, CD253
Mouse IgG1, κ
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Product Citations
Human TRAIL transfected cells stained with RIK-2 PE
  • RIK-2_PE_072007
    Human TRAIL transfected cells stained with RIK-2 PE
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308205 25 tests $129
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308206 100 tests $304
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TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. This antibody is useful for flow cytometric staining and blocking studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
African Green
Antibody Type
Host Species
Human TRAIL-transfected mouse cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308214).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
  2. Uno K, et al. 2003. Blood 101:3658. (Block)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  4. Denny MF, et al. 2007. Blood 110:2907. (Block)
  5. Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
Product Citations
  1. Golinelli G, et al. 2021. Transl Oncol. 15:101240. PubMed
  2. Kalb M, et al. 2012. J Immunol. 188:1583. PubMed
  3. Knörck A, et al. 2022. Front Immunol. 13:838484. PubMed
  4. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  6. Alonso R, et al. 2022. Blood Adv. :. PubMed
  7. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  8. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
  9. Ray JE, et al. 2021. Cancer Biol Ther. 22:554. PubMed
  10. Lulla AR, et al. 2022. Am J Cancer Res. 12:315. PubMed
  11. Choi Y, et al. 2015. Gut. 64: 1303-1313. PubMed
  12. Achard C, et al. 2017. Oncoimmunology. 6:e1261240. PubMed
AB_345291 (BioLegend Cat. No. 308205)
AB_2205825 (BioLegend Cat. No. 308206)

Antigen Details

TNF ligand superfamily member, approximately 30 kD

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.

Gene ID
8743 View all products for this Gene ID
View information about CD253 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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