ELISA MAX™ Standard Set Mouse IL-6

Pricing & Availability
Other Names
IL-6 ELISA Kit
Ave. Rating
11 reviews
Product Citations
publications
mouse il-6_122109
  • mouse il-6_122109
Cat # Size Price Quantity Save
431301 5 plates $225
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-6 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-6 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-6 Standard
  • Avidin-HRP (1000X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Stolk M, et al. 2006. J. Leukoc. Biol. 80:651.
  2. O'Connell PJ, et al. 2006. Blood 107:1010.
  3. Kuroishi T, et al. 2013. Toxicol Sci. 135:119. PubMed
  4. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
Product Citations
  1. Barberi T, et al. 2012. PLoS One. 7:e29808. PubMed
  2. Kuroishi T, et al. 2013. Toxicol Sci. 135:119. PubMed
  3. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  4. Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed
  5. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  6. Hwang J, et al. 2015. Sci Rep. 5: 15971. PubMed
  7. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  8. Orabi A, et al. 2017. Cell Mol Gastroenterol Hepatol. 3(1):119-128. PubMed
  9. Lopez-Millan B, et al. 2017. Exp Mol Med. 49:e290. PubMed
  10. Phillippe M, Adeli S, et al. 2017. PLoS One. 12(6):e0178845. PubMed
  11. Sun H, et al. 2018. J Lipid Res. 59:207. PubMed
  12. Zhao M, et al. 2018. Cell Death Dis. 9:582. PubMed
  13. Zhou P, et al. 2018. Nat Commun. 9:1243. PubMed
Publication Library
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account