FITC anti-mouse H-2D d Antibody

Pricing & Availability
Clone
34-2-12 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (C3H/HeJ) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
34-2-12_FITC_H2Dd_Antibody_FC_1_102813
BALB/c mouse splenocytes were stained with H-2Dd (clone 34-2-12) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).
  • 34-2-12_FITC_H2Dd_Antibody_FC_1_102813
    BALB/c mouse splenocytes were stained with H-2Dd (clone 34-2-12) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).
  • 34-2-12_FITC_H2Dd_Antibody_FC_2_102813
    C57BL/6 mouse splenocytes were stained with H-2Dd (clone 34-2-12) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
110606 500 µg $250
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
(C57BL/6 x DBA/2)F1 mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.

This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. McCluskey J, et al. 1985. Cell 40:247.
  3. McCluskey J, et al. 1986. J. Immunol. 136:1472.
  4. Kane KP. 1994. J. Exp. Med. 179:1011.
  5. Daniels BF, et al. 1994. J. Exp. Med. 180:687.
  6. Nikolic B, et al. 1999. J. Immunol. 162:3402.
  7. Ma XT, et al. 2006. Cancer Research 66:1169.
  8. Matsumoto N, et al. 1998. Immunity 8:245.
Product Citations
  1. Ma X, et al. 2006. Cancer Res. 66:1169. PubMed
Publication Library
RRID
AB_313487 (BioLegend Cat. No. 110606)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Dd haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Dd on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account