- Clone
- 34-2-12 (See other available formats)
- Other Names
- MHC class I
- Isotype
- Mouse (C3H/HeJ) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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BALB/c mouse splenocytes stained with 34-2-12 Alexa Fluor® 647
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 110612 | 100 µg | $195 | ||||
The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.
Product DetailsProduct Details
- Reactivity
- Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- (C57BL/6 x DBA/2)F1 mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC, IF - Reported in the literature - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).
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Application References
(PubMed link indicates BioLegend citation) -
- Ozato K, et al. 1982. Transplantation 34:113.
- McCluskey J, et al. 1985. Cell 40:247.
- McCluskey J, et al. 1986. J. Immunol. 136:1472.
- Kane KP. 1994. J. Exp. Med. 179:1011.
- Daniels BF, et al. 1994. J. Exp. Med. 180:687.
- Nikolic B, et al. 1999. J. Immunol. 162:3402.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Matsumoto N, et al. 1998. Immunity 8:245.
- Product Citations
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- RRID
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AB_492913 (BioLegend Cat. No. 110612)
Antigen Details
- Structure
- MHC class I
- Distribution
- Nucleated cells of the H-2Dd haplotype
- Function
- Antigen presentation
- Ligand Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. - Gene ID
- 14964 View all products for this Gene ID
- UniProt
- View information about H-2Dd on UniProt.org
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