Brilliant Violet 421™ anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_BV421_032911
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 1A8_BV421_032911
    C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 1A8_BV421_Ly-6G_Antibody2_IHC_122916
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS and 5% rat/mouse serum for one hour at room temperature. Then the section was stained with 2.5 µg/mL of Ly-6G (clone 1A8) Brilliant Violet 421™ (red), 5 µg/mL of CD3 Alexa Fluor® 647 (green), and 5 µg/mL of B220 Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.
See Brilliant Violet 421™ spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Avail. Save
127627 125 µl $155
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127628 50 µg $230
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This product is offered in multiple sizing options (µg and µL/test sizes). To compare these sizing options and learn which is the best choice for you, please contact tech support.
Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  2. Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed
  3. Kennedy J, et al. 2014. J Exp Med. 211:2519. PubMed
  4. Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed
  5. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  6. Kullberg M, et al. 2015. Nanomedicine. 11: 1355-1363. PubMed
  7. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  8. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  9. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  10. Linehan JL 2018. Cell. 172:784. PubMed
  11. Macdougall CE 2018. Cell metabolism. 27:588. PubMed
  12. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  13. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  14. Nepal S, et al. 2019. Proc Natl Acad Sci U S A. 116:16513. PubMed
Publication Library
RRID
AB_10897944 (BioLegend Cat. No. 127627)
AB_2562567 (BioLegend Cat. No. 127628)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 5    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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