Alexa Fluor 594 anti-mouse Ly-6G Antibody anti-Ly-6G

Pricing & Availability

Clone: 1A8 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

IA8_A594_Ly-6G_Antibody_1_112520 — C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of Ly-6G (clone 1A8) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (blue), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.

Compare all formats See Alexa Fluor® 594 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

127636

100 µg

$275

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 5.0 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
Moniaga C, et al. 2015. Sci Rep. 5: 15319. PubMed

RRID

AB_2563207 (BioLegend Cat. No. 127636)

Antigen Details

Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644 View all products for this Gene ID
UniProt: View information about Ly-6G on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Immunohistochemistry Protocol for Frozen Sections

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse Ly-6G	1A8	IHC-F
Purified anti-mouse Ly-6G	1A8	FC, IHC-F, IHC
Biotin anti-mouse Ly-6G	1A8	FC, IHC
FITC anti-mouse Ly-6G	1A8	FC
PE anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 647 anti-mouse Ly-6G	1A8	FC, IHC-F
Pacific Blue™ anti-mouse Ly-6G	1A8	FC
APC anti-mouse Ly-6G	1A8	FC
PerCP/Cyanine5.5 anti-mouse Ly-6G	1A8	FC
PE/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 700 anti-mouse Ly-6G	1A8	FC, IHC
APC/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 488 anti-mouse Ly-6G	1A8	FC, IHC-F, SB
Brilliant Violet 421™ anti-mouse Ly-6G	1A8	FC, IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G	1A8	FC
Ultra-LEAF™ Purified anti-mouse Ly-6G	1A8	FC, Depletion, IHC
Brilliant Violet 510™ anti-mouse Ly-6G	1A8	FC
Purified anti-mouse Ly-6G (Maxpar® Ready)	1A8	FC, CyTOF®, WB
Brilliant Violet 650™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 711™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 605™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 785™ anti-mouse Ly-6G	1A8	FC
PE/Dazzle™ 594 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 750 anti-mouse Ly-6G	1A8	FC
PerCP anti-mouse Ly-6G	1A8	FC
TotalSeq™-A0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-C0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-B0015 anti-mouse Ly-6G	1A8	PG
Spark Blue™ 550 anti-mouse Ly-6G	1A8	FC
Spark NIR™ 685 anti-mouse Ly-6G	1A8	FC
Spark YG™ 593 anti-mouse Ly-6G	1A8	FC
PE/Cyanine5 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
Spark UV™ 387 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 640 anti-mouse Ly-6G	1A8	FC

Concentration & Expiration Lookup

Certificate of Analysis

Customers Also Purchased

Alexa Fluor® 488 anti-mouse Ly-6G

Clone: 1A8

Alexa Fluor® 594 anti-mouse F4/80

Clone: BM8

Brilliant Violet 510™ anti-mouse/human CD11b

Clone: M1/70

APC/Cyanine7 anti-mouse MERTK (Mer)

Clone: 2B10C42

Go To Top Version: 4 Revision Date: 11/25/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.