PE anti-mouse H-2D d Antibody

Pricing & Availability
Clone
34-2-12 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (C3H/HeJ) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
34-2-12
BALB/c mouse splenocytes stained with 34-2-12 PE
  • 34-2-12
    BALB/c mouse splenocytes stained with 34-2-12 PE
  • 34-2-12_b
    BALB/c mouse splenocytes stained with 34-2-12 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
110607 50 µg $105
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110608 200 µg $265
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
(C57BL/6 x DBA/2)F1 mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.

This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. McCluskey J, et al. 1985. Cell 40:247.
  3. McCluskey J, et al. 1986. J. Immunol. 136:1472.
  4. Kane KP. 1994. J. Exp. Med. 179:1011.
  5. Daniels BF, et al. 1994. J. Exp. Med. 180:687.
  6. Nikolic B, et al. 1999. J. Immunol. 162:3402.
  7. Ma XT, et al. 2006. Cancer Research 66:1169.
  8. Matsumoto N, et al. 1998. Immunity 8:245.
  9. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  10. Cui Y, et al. 2014. J Immunol. 193:4783. PubMed
Product Citations
  1. Greeneltch K, et al. 2007. Cancer Res. 67:10406. PubMed
  2. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  3. Cui Y, et al. 2014. J Immunol. 193:4783. PubMed
  4. Hirata Y 2018. Cell stem cell. 22:445. PubMed
Publication Library
RRID
AB_313488 (BioLegend Cat. No. 110607)
AB_313489 (BioLegend Cat. No. 110608)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Dd haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Dd on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account