PE anti-mouse H-2D d Antibody

Pricing & Availability
34-2-12 (See other available formats)
Other Names
MHC class I
Mouse (C3H/HeJ) IgG2a, κ
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Product Citations
BALB/c mouse splenocytes stained with 34-2-12 PE
  • 34-2-12
    BALB/c mouse splenocytes stained with 34-2-12 PE
  • 34-2-12_b
    BALB/c mouse splenocytes stained with 34-2-12 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
110607 50 µg $105
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110608 200 µg $265
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The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

Product Details
Technical data sheet

Product Details

Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc
Antibody Type
Host Species
(C57BL/6 x DBA/2)F1 mouse splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.

This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. McCluskey J, et al. 1985. Cell 40:247.
  3. McCluskey J, et al. 1986. J. Immunol. 136:1472.
  4. Kane KP. 1994. J. Exp. Med. 179:1011.
  5. Daniels BF, et al. 1994. J. Exp. Med. 180:687.
  6. Nikolic B, et al. 1999. J. Immunol. 162:3402.
  7. Ma XT, et al. 2006. Cancer Research 66:1169.
  8. Matsumoto N, et al. 1998. Immunity 8:245.
  9. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  10. Cui Y, et al. 2014. J Immunol. 193:4783. PubMed
Product Citations
  1. Greeneltch K, et al. 2007. Cancer Res. 67:10406. PubMed
  2. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  3. Cui Y, et al. 2014. J Immunol. 193:4783. PubMed
  4. Hirata Y 2018. Cell stem cell. 22:445. PubMed
Publication Library
AB_313488 (BioLegend Cat. No. 110607)
AB_313489 (BioLegend Cat. No. 110608)

Antigen Details

MHC class I

Nucleated cells of the H-2Dd haplotype

Antigen presentation
Ligand Receptor
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
View information about H-2Dd on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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