Loading...

Biotin anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
BM8_Biotin_090707
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
  • BM8_Biotin_090707
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
123105 50 µg $59
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123106 500 µg $178
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sasaki O, et al. 2013. J Immunol. 191:2879. PubMed
  2. Knab L, et al. 2014. Mol Cancer Res. 12:1440. PubMed
  3. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  4. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  5. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  6. Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
  7. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  8. Yuan D et al. 2017. Cancer cell. 31(6):771-789 . PubMed
  9. Iturri L, et al. 2021. Immunity. . PubMed
  10. Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed
  11. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  12. Georgilis A et al. 2018. Cancer cell. 34(1):85-102 . PubMed
  13. Xu S, et al. 2016. PLoS One. 11: 0163829. PubMed
  14. Wu W, et al. 2016. PLoS One. 11: 0159512. PubMed
  15. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  16. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  17. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  18. Yauch L, et al. 2010. J Immunol. 185:5405. PubMed
  19. Zoller E, et al. 2011. J Exp Med. 208:1203. PubMed
  20. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  21. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  22. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  23. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  24. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  25. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  26. Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed
  27. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  28. Zeng Z, et al. 2022. Oncogene. :. PubMed
  29. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  30. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  31. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  32. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  33. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  34. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  35. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  36. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  37. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  38. Werner A, et al. 2021. iScience. 24:103076. PubMed
  39. Matsumoto S, et al. 2010. J Immunol. 184:1543. PubMed
  40. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  41. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  42. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  43. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  44. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
  45. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
RRID
AB_893499 (BioLegend Cat. No. 123105)
AB_893501 (BioLegend Cat. No. 123106)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 10/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 605™ anti-mouse F4/80

  • Purified anti-mouse F4/80

  • Biotin anti-mouse F4/80

  • FITC anti-mouse F4/80

  • PE anti-mouse F4/80

  • PE/Cyanine5 anti-mouse F4/80

  • PE/Cyanine7 anti-mouse F4/80

  • APC anti-mouse F4/80

  • APC/Cyanine7 anti-mouse F4/80

  • Alexa Fluor® 488 anti-mouse F4/80

  • Alexa Fluor® 647 anti-mouse F4/80

  • Pacific Blue™ anti-mouse F4/80

  • PerCP anti-mouse F4/80

  • PerCP/Cyanine5.5 anti-mouse F4/80

  • Alexa Fluor® 700 anti-mouse F4/80

  • Brilliant Violet 421™ anti-mouse F4/80

  • Brilliant Violet 510™ anti-mouse F4/80

  • Alexa Fluor® 594 anti-mouse F4/80

  • Brilliant Violet 785™ anti-mouse F4/80

  • Purified anti-mouse F4/80 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse F4/80

  • Brilliant Violet 650™ anti-mouse F4/80

  • Brilliant Violet 711™ anti-mouse F4/80

  • APC/Fire™ 750 anti-mouse F4/80

  • TotalSeq™-A0114 anti-mouse F4/80

  • TotalSeq™-B0114 anti-mouse F4/80

  • TotalSeq™-C0114 anti-mouse F4/80

  • Spark YG™ 570 anti-mouse F4/80

  • KIRAVIA Blue 520™ anti-mouse F4/80

  • Ultra-LEAF™ Purified anti-mouse F4/80

  • APC/Fire™ 810 anti-mouse F4/80

  • Spark NIR™ 685 anti-mouse F4/80

  • Spark Blue™ 550 anti-mouse F4/80
ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account