Biotin anti-mouse NK-1.1 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK-1⁺ cells from mouse spleen and bone marrow

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity³, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis⁶, blocking of NK cell function⁷, induction of proliferation⁸, immunohistochemical staining of frozen sections¹¹, immunofluorescence microscopy¹¹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)

1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

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3. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
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7. Garza-Rodea A, et al. 2011. PLoS One. 6:e14493. PubMed
8. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
9. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
10. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
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12. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
13. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
14. Wang T, et al. 2020. Blood Sci. 2:79. PubMed
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25. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
26. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
27. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
28. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
29. Shen Z, et al. 2021. iScience. 24:103014. PubMed
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32. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
33. Dong S, et al. 2021. Nature. 591:117. PubMed
34. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
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37. Yao M, et al. 2020. Cell. 180(3):502-520. PubMed
38. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
39. Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed
40. Zeng Z, et al. 2022. Oncogene. :. PubMed
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44. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
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49. Zeis P, et al. 2020. Immunity. 53:775. PubMed
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51. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
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RRID

AB_313390 (BioLegend Cat. No. 108703)
AB_313391 (BioLegend Cat. No. 108704)

Antigen Details

Structure

NKR-P1 gene family

Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function

NK cell activation, IFN-γ production, cytotoxic granule release

Cell Type

NK cells, NKT cells

Biology Area

Immunology, Innate Immunity

Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID

17059 View all products for this Gene ID

UniProt

View information about NK-1.1 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?

We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

Certificate of Analysis