Alexa Fluor® 488 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
4 reviews
Product Citations
publications
BM8_A488_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_2_IHCF_060616
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 488 (green), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 510™ (blue), and anti-mouse CD3 (clone 17A2) Brilliant Violet 421™ (red) overnight at 4°C. The image was captured with a 10X objective.
See Alexa Fluor® 488 spectral data See high resolution IHC data...
Cat # Size Price Quantity Avail. Save
123119 25 µg $125
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123120 100 µg $275
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Ohnishi A, et al. 2015. PLoS One. 10:117616. PubMed
Product Citations
  1. Bian Z, et al. 2012. J Immunol. 188:844. PubMed
  2. Caruso R, et al. 2009. Gastroenterology. 136:2270. PubMed
  3. Carlock C, et al. 2013. Reproduction. 146:491. PubMed
  4. Dong Z, et al. 2013. PLoS One. 18:80364. PubMed
  5. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  6. Carlock C, et al. 2014. J Immunol. 193:161. PubMed
  7. Care A, et al. 2014. Biol Reprod. 91:60. PubMed
  8. Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed
  9. Ohnishi A, et al. 2015. PLoS One. 10:117616. PubMed
  10. Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed
  11. Wheeler R, Gull E 2015. Nat Commun. 6: 8964. PubMed
  12. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  13. Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed
  14. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  15. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  16. Niu S, et al. 2016. J Immunol. 197: 3293 - 3301. PubMed
  17. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  18. Wang H, et al. 2016. PLoS One. 11:e0167307. PubMed
  19. Yi-Wei Lin, Emmanuel Montassier, Dan Knights 2016. Sci Rep. 6:38599. PubMed
  20. Chakraborty K, et al. 2017. Nat Commun. 8:13944. PubMed
  21. Antsiferova M, et al. 2017. EMBO Mol Med. 9(1):27-45. PubMed
  22. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  23. Tedford K, et al. 2017. Nat Commun. . 10.1038/s41467-017-02482-4. PubMed
  24. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  25. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  26. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  27. Lin JR 2018. eLife. 7: e31657. PubMed
  28. Veremeyko T 2018. Journal of neuroscience research. 97:162. PubMed
Publication Library
RRID
AB_893491 (BioLegend Cat. No. 123119)
AB_893479 (BioLegend Cat. No. 123120)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution
Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function
Induction of immunological tolerance
Cell Type
Macrophages, Langerhans cells, Dendritic cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

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