Alexa Fluor® 488 anti-mouse I-Ab (Aβb) Antibody

Pricing & Availability
25-9-17 (See other available formats)
Other Names
MHC class II
Mouse (C3H) IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with 25-9-17 Alexa Fluor® 488
  • 25-9-17_Alx488_122607
    C57BL/6 mouse splenocytes stained with 25-9-17 Alexa Fluor® 488
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
114407 25 µg $85
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The 25-9-17 antibody reacts with the beta chain of the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2d, p, q haplotypes.

Product Details
Technical data sheet

Product Details

Mouse b haplotype, Cross-reacts with alloantigens of H-2d,p,q haplotypes
Antibody Type
Host Species
C3H.SW splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.

Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (IP)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747.
  3. Ozato K, et al. 1981. J. Immunol. 126:317.
  4. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC)
  5. Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC)
  6. Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed
  7. Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed.
  8. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.
AB_493144 (BioLegend Cat. No. 114407)

Antigen Details

ß chain of I-Ab MHC class II

B cell and activated T cells, APCs of H-2b mice

Antigen presentation
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
View information about I-Ab on

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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