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Ultra-LEAF™ Purified anti-mouse CD25 Antibody

Pricing & Availability
Clone
A18246G (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_A18246G_ULEAF_CD25_Antibody_1_061323
250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clone A18246G) (left) or 250 µg Ultra-LEAF™ Purified rat IgG2b, κ isotype control (clone RTK4530) (right) were intraperitoneally (IP) injected to C57BL/6 mice, and splenocytes analyzed on day 7 post-injection. Cells shown are gated CD3+ splenocyte population.
  • A_A18246G_ULEAF_CD25_Antibody_1_061323
    250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clone A18246G) (left) or 250 µg Ultra-LEAF™ Purified rat IgG2b, κ isotype control (clone RTK4530) (right) were intraperitoneally (IP) injected to C57BL/6 mice, and splenocytes analyzed on day 7 post-injection. Cells shown are gated CD3+ splenocyte population.
  • B_A18246G_ULEAF_CD25_Antibody_2_061323
    % CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneally injected with 250 µg Ultra-LEAF™ Rat IgG2b, κ Isotype Ctrl (clone RTK4530) (black line) or Ultra-LEAF™ anti-mouse CD25 (clone A18246G) (purple line) at indicated days post-injection. Error bars represent standard deviation from 3 mice analyzed per condition and timepoint.
Cat # Size Price Quantity Check Availability Save
112603 100 µg £143
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112604 1 mg £411
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Description

CD25, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac, is a 55 kD glycoprotein. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For in vivo depletion, suggested use is 250 µg of Ultra-LEAF™ purified of clone A18246G for one C57BL/6 mouse intraperitoneal injection. It is recommended that the reagent be titrated for optimal performance.

Additional Product Notes

Ultra-LEAF™ purified (clone A18246G) antibody recognizes a different epitope compared to other mouse CD25 such as clones 3C7 and PC61.

Ultra-LEAF™ purified (clone A18246G) antibody can deplete CD25+ cells in vivo in C57BL/6 mouse by intraperitoneal injection.

RRID
AB_2941391 (BioLegend Cat. No. 112603)
AB_2941391 (BioLegend Cat. No. 112604)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell. 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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