- TS1/18 (See other available formats)
- V AS162
- Other Names
- Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
- Mouse IgG1, κ
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- Product Citations
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CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.Product Details
- Human, Cross-Reactivity: Swine (Pig, Porcine)
- Antibody Type
- Host Species
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302112). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
- Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
- Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
- Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
AB_2561483 (BioLegend Cat. No. 302116)
- Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
- Adhesion and signaling in hematopoietic system
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.
- Gene ID
- 3689 View all products for this Gene ID
- View information about CD18 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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