Ultra-LEAF™ Purified anti-mouse/rat CD29 Antibody

Pricing & Availability
Clone
HMβ1-1 (See other available formats)
Other Names
integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
hmb1-1.jpg
C57BL/6 mouse splenocytes stained with HMβ1-1 PE.
  • hmb1-1.jpg
    C57BL/6 mouse splenocytes stained with HMβ1-1 PE.
  • hmb1-2.jpg
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
Cat # Size Price Quantity Avail. Save
102235 100 µg £144
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102236 1 mg £420
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Description

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC-F, IP, Block - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 102235 and 102236).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
  3. Jia W, et al. 2005. Blood 106:3854.PubMed
  4. Economopoulou M, et al. 2005. Blood 106:3831.
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  8. Kim DT, et al. 2008. Blood 111:2929.PubMed
  9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
  11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
RRID
AB_2813927 (BioLegend Cat. No. 102235)
AB_2813928 (BioLegend Cat. No. 102236)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/15/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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