Spark Violet™ 423 anti-human CD123 Antibody

Pricing & Availability
S18016F (See other available formats)
Regulatory Status
Other Names
IL-3Rα, IL-3 Receptor alpha
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with anti-human CD45RO APC and anti-human CD123 (clone S18016F) Spark Violet™ 423 (left) or CD45RO APC only (right).
  • S18016F_SPKVL423_CD123_Antibody_121421
    Human peripheral blood lymphocytes were stained with anti-human CD45RO APC and anti-human CD123 (clone S18016F) Spark Violet™ 423 (left) or CD45RO APC only (right).
Compare all formats See Spark Violet™ 423 spectral data
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396719 25 tests £94
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CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Hu CD123 transfectants
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
AB_2910457 (BioLegend Cat. No. 396719)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD.

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes.

Hematopoietic cell proliferation, differentiation
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Miyajima A, et al. 1993. Blood. 82:1960.
Gene ID
3563 View all products for this Gene ID
View information about CD123 on

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Go To Top Version: 1    Revision Date: 11/18/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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