Loading...

PE/Fire™ 810 anti-human CD123 Antibody

Pricing & Availability
Clone
S18016F (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
S18016F_PE-Fire810 _CD123_Antibody_updated_032023
Human peripheral blood lymphocytes stained with anti-human CD45RO Brilliant Violet 421™ and anti-human CD123 (clone S18016F) PE/Fire™ 810 (left) or stained with anti-human CD45RO Brilliant Violet 421™ only (right).
  • S18016F_PE-Fire810 _CD123_Antibody_updated_032023
    Human peripheral blood lymphocytes stained with anti-human CD45RO Brilliant Violet 421™ and anti-human CD123 (clone S18016F) PE/Fire™ 810 (left) or stained with anti-human CD45RO Brilliant Violet 421™ only (right).
Compare all formats See PE/Fire™ 810 spectral data
Cat # Size Price Quantity Check Availability Save
396723 25 tests £117
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hu CD123 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2936599 (BioLegend Cat. No. 396723)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD.
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes.

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Miyajima A, et al. 1993. Blood. 82:1960.
Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/13/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD123

  • Purified anti-human CD123

  • KIRAVIA Blue 520™ anti-human CD123

  • PE/Dazzle™ 594 anti-human CD123

  • APC anti-human CD123

  • APC/Fire™ 750 anti-human CD123 Antibody

  • PE/Cyanine7 anti-human CD123 Antibody

  • PerCP/Cyanine5.5 anti-human CD123 Antibody

  • Spark NIR™ 685 anti-human CD123

  • Spark Violet™ 423 anti-human CD123 Antibody

  • Biotin anti-human CD123

  • PE/Fire™ 810 anti-human CD123
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account