Spark Blue™ 550 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_SPKBL_550_CD3_Antibody_100219
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) Spark Blue™ 550 (filled histogram). Open histogram represents unstained cells.
  • 17A2_SPKBL_550_CD3_Antibody_100219
    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) Spark Blue™ 550 (filled histogram). Open histogram represents unstained cells.
Cat # Size Price Quantity Avail. Save
100259 25 µg £81
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions. The solution is free of unconjugated Spark Blue™ 550.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 550 nm

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/02/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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