Ultra-LEAF Purified anti-mouse CD3 Antibody anti-CD3

Pricing & Availability

Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD3 (clone 17A2), followed by anti-rat IgG FITC.

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100239

100 µg

£76

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100238

1 mg

£102

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100253

5 mg

£369

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100254

25 mg

£964

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100255

50 mg

£1611

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100256

100 mg

£2314

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This product is eligible for a 40% discount! Promotion valid until December 31, 2023. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical Data Sheet (pdf)

Product Details

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References (PubMed link indicates BioLegend citation)

Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed
Saito S, et al. 2020. Nutrients. 12:. PubMed
Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
Fajstova A, et al. 2020. Cells. 9:00. PubMed
Watson MJ, et al. 2021. Nature. 591:645. PubMed
Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
De Cicco P, et al. 2019. British Journal of Pharmacology. 177(4):884-897. PubMed
Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
Xu L, et al. 2022. Nat Commun. 13:6881. PubMed
Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed

RRID

AB_2810313 (BioLegend Cat. No. 100239)
AB_2561487 (BioLegend Cat. No. 100238)
AB_2810314 (BioLegend Cat. No. 100253)
AB_2810315 (BioLegend Cat. No. 100254)
AB_2810316 (BioLegend Cat. No. 100255)
AB_2810317 (BioLegend Cat. No. 100256)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD3	17A2	FC
PE anti-mouse CD3	17A2	FC
Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Alexa Fluor® 488 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3	17A2	FC
Alexa Fluor® 700 anti-mouse CD3	17A2	FC
PerCP/Cyanine5.5 anti-mouse CD3	17A2	FC
PE/Cyanine7 anti-mouse CD3	17A2	FC
APC/Cyanine7 anti-mouse CD3	17A2	FC
Brilliant Violet 421™ anti-mouse CD3	17A2	FC,ICC
Brilliant Violet 570™ anti-mouse CD3	17A2	FC
Brilliant Violet 650™ anti-mouse CD3	17A2	FC
Brilliant Violet 785™ anti-mouse CD3	17A2	FC
Brilliant Violet 510™ anti-mouse CD3	17A2	FC
APC anti-mouse CD3	17A2	FC
Ultra-LEAF™ Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3	17A2	FC
Alexa Fluor® 594 anti-mouse CD3	17A2	IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3	17A2	FC
Biotin anti-mouse CD3	17A2	FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3	17A2	FC
APC/Fire™ 750 anti-mouse CD3	17A2	FC
Brilliant Violet 750™ anti-mouse CD3	17A2	FC
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2	FC
Spark NIR™ 685 anti-mouse CD3	17A2	FC
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2	FC
PE/Fire™ 640 anti-mouse CD3	17A2	FC
Spark YG™ 570 anti-mouse CD3	17A2	IHC-F
PE/Fire™ 700 anti-mouse CD3	17A2	FC
PE/Cyanine5 anti-mouse CD3	17A2	FC
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2	FC
Spark Violet™ 423 anti-mouse CD3	17A2	FC
PE/Fire™ 810 anti-mouse CD3	17A2	FC
Spark Red™ 718 anti-mouse CD3	17A2	FC
Spark UV™ 387 anti-mouse CD3	17A2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.