Purified anti-mouse CD11c Maxpar Ready Antibody anti-CD11c

Pricing & Availability

Clone: N418 (See other available formats)
Regulatory Status: RUO
Other Names: αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype: Armenian Hamster IgG
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Product Citations: publications

N418_Purified_CD11c_CyTOF_112113 — Mouse splenocytes stained with 147Sm-anti-CD45 (30-F11) and 142Nd-anti-CD11c (N418). Data provided by DVS Sciences.

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117341

100 µg

£73

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Description

CD11c is a 150 kD glycoprotein also known as α_X integrin, CR4, and p150. CD11c forms a α_Xβ₂ heterodimer with β₂ integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The α_Xβ₂ integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse spleen dendritic cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen sections³, immunofluorescence microscopy^{5, 9} (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections¹⁰), and spatial biology (IBEX)^22,23.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Granucci F, et al. 1997. J. Immunol. 159:1794.
Stokes RW, et al. 1998. J. Immunol. 160:5514.
Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
Ma XT, et al. 2006. Cancer Research 66:1169.
Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
White CE, et al. 2015. J Immunol. 194:697. PubMed
Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
Wei SC, et al. 2019. Immunity. 50:1084. PubMed
Janela B, et al. 2019. Immunity. 50:1069. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2562807 (BioLegend Cat. No. 117341)

Antigen Details

Structure: Integrin α-chain, associates with integrin β₂ (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand/Receptor: iC3b, fibrinogen
Cell Type: Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
Gene ID: 16411 View all products for this Gene ID
UniProt: View information about CD11c on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD11c Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD11c	N418	FC
Biotin anti-mouse CD11c	N418	FC,IHC-F
FITC anti-mouse CD11c	N418	FC
PE anti-mouse CD11c	N418	FC
Purified anti-mouse CD11c	N418	FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c	N418	FC
Alexa Fluor® 647 anti-mouse CD11c	N418	FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c	N418	FC
PE/Cyanine7 anti-mouse CD11c	N418	FC
Brilliant Violet 605™ anti-mouse CD11c	N418	FC
Alexa Fluor® 700 anti-mouse CD11c	N418	FC
Pacific Blue™ anti-mouse CD11c	N418	FC
APC/Cyanine7 anti-mouse CD11c	N418	FC
PerCP/Cyanine5.5 anti-mouse CD11c	N418	FC
PerCP anti-mouse CD11c	N418	FC
Brilliant Violet 421™ anti-mouse CD11c	N418	FC,IHC
Brilliant Violet 570™ anti-mouse CD11c	N418	FC
Brilliant Violet 785™ anti-mouse CD11c	N418	FC
Brilliant Violet 510™ anti-mouse CD11c	N418	FC
Brilliant Violet 650™ anti-mouse CD11c	N418	FC
Purified anti-mouse CD11c (Maxpar® Ready)	N418	FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c	N418	IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c	N418	FC
Brilliant Violet 711™ anti-mouse CD11c	N418	FC
APC/Fire™ 750 anti-mouse CD11c	N418	FC
TotalSeq™-A0106 anti-mouse CD11c	N418	PG
Brilliant Violet 750™ anti-mouse CD11c	N418	FC
TotalSeq™-B0106 anti-mouse CD11c	N418	PG
TotalSeq™-C0106 anti-mouse CD11c	N418	PG
KIRAVIA Blue 520™ anti-mouse CD11c	N418	FC
Spark Blue™ 550 anti-mouse CD11c	N418	FC
Spark NIR™ 685 anti-mouse CD11c	N418	FC
Spark UV™ 387 anti-mouse CD11c	N418	FC
Spark Red™ 718 anti-mouse CD11c	N418	FC
Spark Blue™ 515 anti-mouse CD11c	N418	FC
PerCP/Fire™ 806 anti-mouse CD11c	N418	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07/21/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.