Purified anti-mouse CD8a Maxpar Ready Antibody anti-CD8a

Pricing & Availability

Clone: 53-6.7 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat IgG2a, κ
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Product Citations: publications

53-6.7_Purified_CD8a_CyTOF_112113 — C57BL/6 mouse splenocytes stained with 147Sm-anti-CD45 (30-F11) and 168Er-anti-CD8a (53-6.7). Data provided by DVS Sciences.

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100755

100 µg

£57

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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes³. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a⁺ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation^1,3, in vivo and in vitro cell depletion^2,10,15, inhibition of CD8 T cell proliferation³, blocking of cytotoxicity^3,4, immunohistochemical staining^5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Kamimura D, et al. 2006. J. Immunol. 177:306.
Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
Lee CH, et al. 2009. Clin. Cancer Res. PubMed
Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
Guo Y, et al. 2008. Blood 112:480. PubMed
Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
Kenna TJ, et al. 2008. Blood 111:2091. PubMed
Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Medyouf H, et al. 2010. Blood 115:1175. PubMed
Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Cui L, et al. 2015. J Control Release. 206:220. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2562796 (BioLegend Cat. No. 100755)

Antigen Details

Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525 View all products for this Gene ID
UniProt: View information about CD8a on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD8a Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD8a	53-6.7	FC
Biotin anti-mouse CD8a	53-6.7	FC,IHC
FITC anti-mouse CD8a	53-6.7	FC
PE anti-mouse CD8a	53-6.7	FC
PE/Cyanine5 anti-mouse CD8a	53-6.7	FC
Purified anti-mouse CD8a	53-6.7	FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a	53-6.7	FC
APC/Cyanine7 anti-mouse CD8a	53-6.7	FC
Alexa Fluor® 488 anti-mouse CD8a	53-6.7	FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a	53-6.7	FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a	53-6.7	FC
Alexa Fluor® 700 anti-mouse CD8a	53-6.7	FC
PerCP/Cyanine5.5 anti-mouse CD8a	53-6.7	FC
PerCP anti-mouse CD8a	53-6.7	FC
Brilliant Violet 421™ anti-mouse CD8a	53-6.7	FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 650™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 605™ anti-mouse CD8a	53-6.7	FC
Ultra-LEAF™ Purified anti-mouse CD8a	53-6.7	FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 785™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 510™ anti-mouse CD8a	53-6.7	FC
Purified anti-mouse CD8a (Maxpar® Ready)	53-6.7	FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a	53-6.7	IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a	53-6.7	FC
APC/Fire™ 750 anti-mouse CD8a	53-6.7	FC
GoInVivo™ Purified anti-mouse CD8a	53-6.7	FC
TotalSeq™-A0002 anti-mouse CD8a	53-6.7	PG
Spark Blue™ 550 anti-mouse CD8a	53-6.7	FC
Spark NIR™ 685 anti-mouse CD8a	53-6.7	FC
TotalSeq™-C0002 anti-mouse CD8a	53-6.7	PG
TotalSeq™-B0002 anti-mouse CD8a	53-6.7	PG
Spark YG™ 570 anti-mouse CD8a	53-6.7	IHC-F
PE/Fire™ 640 anti-mouse CD8a	53-6.7	FC
PE/Fire™ 700 anti-mouse CD8a	53-6.7	FC
Spark Blue™ 574 anti-mouse CD8a Antibody	53-6.7	FC
Spark Violet™ 423 anti-mouse CD8a Antibody	53-6.7	FC
Spark UV™ 387 anti-mouse CD8a	53-6.7	FC
Spark Blue™ 515 anti-mouse CD8a	53-6.7	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 07/21/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.